| http://purl.uniprot.org/citations/12895521 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12895521 | http://www.w3.org/2000/01/rdf-schema#comment | "The inferior colliculus (IC) plays a key role in the processing of auditory information and is thought to be an important site for genesis of wild running seizures that evolve into tonic-clonic seizures. IC neurons are known to have Ca(2+) channels but neither their types nor their pharmacological properties have been as yet characterized. Here, we report on biophysical and pharmacological properties of Ca(2+) channel currents in acutely dissociated neurons of adult rat IC, using electrophysiological and molecular techniques. Ca(2+) channels were activated by depolarizing pulses from a holding potential of -90 mV in 10 mV increments using 5 mM barium (Ba(2+)) as the charge carrier. Both low (T-type, VA) and high (HVA) threshold Ca(2+) channel currents that could be blocked by 50 microM cadmium, were recorded. Pharmacological dissection of HVA currents showed that nifedipine (10 microM, L-type channel blocker), omega-conotoxin GVIA (1 microM, N-type channel blocker), and omega-agatoxin TK (30 nM, P-type channel blocker) partially suppressed the current by 21%, 29% and 22%, respectively. Since at higher concentration (200 nM) omega-agatoxin TK also blocks Q-type channels, the data suggest that Q-type Ca(2+) channels carry approximately 16% of HVA current. The fraction of current (approximately 12%) resistant to the above blockers, which was blocked by 30 microM nickel and inactivated with tau of 15-50 ms, was considered as R-type Ca(2+) channel current. Consistent with the pharmacological evidences, Western blot analysis using selective Ca(2+) channel antibodies showed that IC neurons express Ca(2+) channel alpha(1A), alpha(1B), alpha(1C), alpha(1D), and alpha(1E) subunits. We conclude that IC neurons express functionally all members of HVA Ca(2+) channels, but only a subset of these neurons appear to have developed functional LVA channels."xsd:string |
| http://purl.uniprot.org/citations/12895521 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0306-4522(03)00323-3"xsd:string |
| http://purl.uniprot.org/citations/12895521 | http://purl.uniprot.org/core/author | "Morad M."xsd:string |
| http://purl.uniprot.org/citations/12895521 | http://purl.uniprot.org/core/author | "N'Gouemo P."xsd:string |
| http://purl.uniprot.org/citations/12895521 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12895521 | http://purl.uniprot.org/core/name | "Neuroscience"xsd:string |
| http://purl.uniprot.org/citations/12895521 | http://purl.uniprot.org/core/pages | "815-826"xsd:string |
| http://purl.uniprot.org/citations/12895521 | http://purl.uniprot.org/core/title | "Voltage-gated calcium channels in adult rat inferior colliculus neurons."xsd:string |
| http://purl.uniprot.org/citations/12895521 | http://purl.uniprot.org/core/volume | "120"xsd:string |
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