| http://purl.uniprot.org/citations/12896982 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12896982 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12896982 | http://www.w3.org/2000/01/rdf-schema#comment | "C to U editing of the nuclear apolipoprotein B (apoB) transcript is mediated by a core enzyme containing a catalytic deaminase, apobec-1, and an RNA binding subunit, apobec-1 complementation factor (ACF). ACF expression is predominantly nuclear, including mutant proteins with deletions of a putative nuclear localization signal. We have now identified a novel 41-residue motif (ANS) in the auxiliary domain of ACF that functions as an authentic nuclear localization signal. ANS-green fluorescence protein and ANS-beta-galactosidase chimeras were both expressed exclusively in the nucleus, whereas wild-type chimeras or an ACF deletion mutant lacking the ANS were cytoplasmic. Nuclear accumulation of ACF is transcription-dependent, temperature-sensitive, and reversible, features reminiscent of a shuttling protein. ACF relocates to the cytoplasm after actinomycin D treatment, an effect blocked by the CRM1 inhibitor leptomycin B. Heterokaryon assays confirmed directly that ACF shuttles in vivo. ACF binds to the protein carrier, transportin 2 in vivo, and colocalizes to the nucleus as determined by confocal microscopy. Co-immunoprecipitation experiments revealed that transportin 2 binds directly to the ANS motif. These data suggest that directed nuclear localization and compartmentalization of the core complex of the apoB RNA editing enzyme is regulated through a dominant targeting sequence (ANS) contained within ACF."xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m302951200"xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m302951200"xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/author | "Kennedy S."xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/author | "Kennedy S."xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/author | "Blanc V."xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/author | "Blanc V."xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/author | "Davidson N.O."xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/author | "Davidson N.O."xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/pages | "41198-41204"xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/pages | "41198-41204"xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/title | "A novel nuclear localization signal in the auxiliary domain of apobec-1 complementation factor regulates nucleocytoplasmic import and shuttling."xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/title | "A novel nuclear localization signal in the auxiliary domain of apobec-1 complementation factor regulates nucleocytoplasmic import and shuttling."xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12896982 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12896982 |
| http://purl.uniprot.org/citations/12896982 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12896982 |
| http://purl.uniprot.org/citations/12896982 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12896982 |
| http://purl.uniprot.org/citations/12896982 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12896982 |