| http://purl.uniprot.org/citations/12898250 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12898250 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12898250 | http://www.w3.org/2000/01/rdf-schema#comment | "Gelatinase A, also called matrix metalloproteinase 2 (MMP-2), belongs to the matrix metalloproteinase (MMP) family. MMP-2 cleaves type IV collagen, denatured collagen (gelatin), and other extracellular matrix (ECM) components. MMP-2 has been reported to be involved in a number of biological and pathological processes, but previous studies have not indicated that its expression is essential for early embryogenesis. In the current study, we have utilized zebrafish as a developmental model to study the role of MMP-2 during embryogenesis. We have successfully isolated a zebrafish MMP-2 (zMMP-2) homologue showing over 80% identity and over 90% similarity to its human counterpart. In situ analysis showed that zMMP-2 was expressed as early as the one-cell stage implying a maternal origin during oogenesis, and embryos continued to express zMMP-2 through at least the 72-h stage of development. RT-PCR analysis confirmed the in situ expression pattern and gelatin zymography indicated that a metalloproteinase with the same gel mobility as vertebrate MMP-2 was present in zebrafish embryos. Injection of zMMP-2 antisense morpholino oligonucleotides into 1-to 4-cell embryos resulted in a truncated axis, monitored through 72 h of development indicating that this metalloproteinase plays an important role in zebrafish embryogenesis. Monpholino-induced alterations in development began to be observed at 12 h of embryogenesis based on morphological and axis marker studies. The results obtained in zebrafish are in contrast to murine knockout studies that indicate that MMP-2 does not have a major role in mouse embryogenesis."xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00427-003-0346-4"xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00427-003-0346-4"xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/author | "Zhang X."xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/author | "Zhang X."xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/author | "Bai S."xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/author | "Bai S."xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/author | "Nagase H."xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/author | "Nagase H."xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/author | "Sarras M.P. Jr."xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/author | "Sarras MP J.r."xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/name | "Dev. Genes Evol."xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/name | "Dev Genes Evol"xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/pages | "456-463"xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/pages | "456-463"xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/title | "The expression of gelatinase A (MMP-2) is required for normal development of zebrafish embryos."xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/title | "The expression of gelatinase A (MMP-2) is required for normal development of zebrafish embryos."xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/volume | "213"xsd:string |
| http://purl.uniprot.org/citations/12898250 | http://purl.uniprot.org/core/volume | "213"xsd:string |