| http://purl.uniprot.org/citations/12904583 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12904583 | http://www.w3.org/2000/01/rdf-schema#comment | "A major challenge of the postgenomic era is the functional characterization of every single gene within the mammalian genome. In an effort to address this challenge, we assembled a collection of mutations in mouse embryonic stem (ES) cells, which is the largest publicly accessible collection of such mutations to date. Using four different gene-trap vectors, we generated 5,142 sequences adjacent to the gene-trap integration sites (gene-trap sequence tags; http://genetrap.de) from >11,000 ES cell clones. Although most of the gene-trap vector insertions occurred randomly throughout the genome, we found both vector-independent and vector-specific integration "hot spots." Because >50% of the hot spots were vector-specific, we conclude that the most effective way to saturate the mouse genome with gene-trap insertions is by using a combination of gene-trap vectors. When a random sample of gene-trap integrations was passaged to the germ line, 59% (17 of 29) produced an observable phenotype in transgenic mice, a frequency similar to that achieved by conventional gene targeting. Thus, gene trapping allows a large-scale and cost-effective production of ES cell clones with mutations distributed throughout the genome, a resource likely to accelerate genome annotation and the in vivo modeling of human disease."xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1633296100"xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://purl.uniprot.org/core/author | "Arnold H.H."xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://purl.uniprot.org/core/author | "Wurst W."xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://purl.uniprot.org/core/author | "Hansen J."xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://purl.uniprot.org/core/author | "von Melchner H."xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://purl.uniprot.org/core/author | "Schnutgen F."xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://purl.uniprot.org/core/author | "Vauti F."xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://purl.uniprot.org/core/author | "Floss T."xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://purl.uniprot.org/core/author | "Ruiz P."xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://purl.uniprot.org/core/author | "Fuchtbauer E.M."xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://purl.uniprot.org/core/author | "Van Sloun P."xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12904583 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://purl.uniprot.org/core/pages | "9918-9922"xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://purl.uniprot.org/core/title | "A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome."xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://purl.uniprot.org/core/volume | "100"xsd:string |
| http://purl.uniprot.org/citations/12904583 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12904583 |
| http://purl.uniprot.org/citations/12904583 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12904583 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YVV5-mappedCitation-12904583 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12904583 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YVV8-mappedCitation-12904583 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12904583 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YVX6-mappedCitation-12904583 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12904583 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YVY1-mappedCitation-12904583 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12904583 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YVY2-mappedCitation-12904583 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12904583 |