| http://purl.uniprot.org/citations/12907679 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12907679 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12907679 | http://www.w3.org/2000/01/rdf-schema#comment | "The bestrophins are a newly described family of anion channels unrelated in primary sequence to any previously characterized channel proteins. The human genome codes for four bestrophins, each of which confers a distinctive plasma membrane conductance on transfected 293 cells. Extracellular treatment with methanethiosulfonate ethyltrimethylammonium (MTSET) of a series of substitution mutants that eliminate one or more cysteines from human bestrophin1 demonstrates that cysteine 69 is the single endogenous cysteine responsible for MTSET inhibition of whole-cell current. Cysteines introduced between positions 78-99 and 223-226 are also accessible to external MTSET, with MTSET modification at positions 79, 80, 83, and 90 producing a 2-6-fold increase in whole-cell current. The latter set of four cysteine-substitution mutants define a region that appears to mediate allosteric control of channel activity. Mapping of transmembrane topography by insertion of N-linked glycosylation sites and tobacco etch virus protease cleavage sites provides evidence for cytosolic N and C termini and an unexpected transmembrane topography with at least three extracellular loops that include positions 60-63, 212-227, and 261-267. These experiments provide the first structural analysis of the bestrophin channel family."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m306150200"xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m306150200"xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.M306150200"xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/author | "Sun H."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/author | "Sun H."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/author | "Williams J."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/author | "Williams J."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/author | "Nathans J."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/author | "Nathans J."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/author | "Yau K.W."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/author | "Cahill H."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/author | "Cahill H."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/author | "Smallwood P."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/author | "Smallwood P."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/author | "Tsunenari T."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/author | "Tsunenari T."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/author | "Yau K.-W."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/author | "Yau K.-W."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12907679 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |