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http://purl.uniprot.org/citations/12911421http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12911421http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12911421http://www.w3.org/2000/01/rdf-schema#comment"

Background

The group 1 mite allergens are the most significant indoor allergens and they belong to the papain-like cysteine protease family. To date there is only one published report on the isolation and characterization of group 1 allergens from Blomia tropicalis mites. The aims of the study are to determine the cross-reactivity between group 1 allergens and to evaluate their clinical importance in allergic patients.

Methods

The full-length Blo t 1 gene was obtained by SMART RACE cDNA amplification method using gene-specific primers. The sequence alignment was performed using LOOK followed by three-dimensional homology modeling. The cDNA was expressed in Pichia pastoris as a secretory protein. Identification of native Blo t 1 in crude mite and spent mite medium extracts was done by Western immunoblot using monoclonal antibody. Allergenicity of recombinant Blo t 1 and native Der p 1 was examined by human IgE ELISA with 80 asthmatic sera.

Results

The cDNA sequence consists of 1105 base pairs, including 5'- and 3'-untranslating regions, encoding an open reading frame of 330 amino acid residues. The predicted molecular weight of the deduced protein was approximately 38 kDa. Blo t 1 shared 53 and 34% nucleotide and amino acid, respectively, sequence homology with Der p 1. Native Blo t 1 was detected in both crude mite and spent mite medium extracts, and its estimated molecular weight was about 26 kDa. The recombinant Blo t 1 reacted positively with IgE in 90 and 65% of sera from asthmatic children and adults, respectively, indicating that it is a major allergen. The correlation of human IgE reactivity between Blo t 1 and Der p 1 was low in these sera.

Conclusion

The full-length cDNA encoding group 1 Blomia tropicalis mite allergen (designated as Blo t 1) has been characterized and expressed from local mites in Singapore. This fecal allergen showed high frequency of human IgE reactivity with asthmatic sera in the tropics and there was a low correlation of IgE reactivity between Blo t 1 and Der p 1."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.org/dc/terms/identifier"doi:10.1034/j.1398-9995.2003.00215.x"xsd:string
http://purl.uniprot.org/citations/12911421http://purl.org/dc/terms/identifier"doi:10.1034/j.1398-9995.2003.00215.x"xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Kolatkar P.R."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Kolatkar P.R."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Cheong N."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Cheong N."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Lee B.W."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Lee B.W."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Chua K.Y."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Chua K.Y."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Kuo I.C."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Kuo I.C."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Kolortkar P.R."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Kolortkar P.R."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Ramos J.D."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Ramos J.D."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Soon S.C."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/author"Soon S.C."xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/date"2003"xsd:gYear
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/date"2003"xsd:gYear
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/name"Allergy"xsd:string
http://purl.uniprot.org/citations/12911421http://purl.uniprot.org/core/name"Allergy"xsd:string
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