| http://purl.uniprot.org/citations/12912986 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12912986 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12912986 | http://www.w3.org/2000/01/rdf-schema#comment | "N-Myristoylation is an irreversible modification that affects the membrane binding properties of crucial cytoplasmic proteins from signal transduction cascades. We characterized the two putative N-myristoyltransferases of Arabidopsis thaliana as a means of investigating the entire N-myristoylation proteome (N-myristoylome) in a higher eukaryote. AtNMT1 compensated for the nmt1 defect in yeast, whereas AtNMT2 and chimeras of the two genes did not. Only AtNMT1 modified known N-myristoylated proteins in vitro. AtNMT1 is therefore responsible for the A. thaliana N-myristoylome, whereas AtNMT2 does not seem to have usual myristoylation activity. We began with the whole set of N-myristoylated G proteins in the A. thaliana proteome. We then used a reiterative approach, based on the in vitro N-myristoylation of more than 60 different polypeptides, to determine the substrate specificity of AtNMT1. We found that the positive charge on residue 7 of the substrate was particularly important in substrate recognition. The A. thaliana N-myristoylome consists of 437 proteins, accounting for 1.7% of the complete proteome. We demonstrated the N-myristoylation of several unexpected protein families, including innate immunity proteins, thioredoxins, components of the protein degradation pathway, transcription factors, and a crucial regulatory enzyme of glycolysis. The role of N-myristoylation is discussed in each case; in particular, this process may underlie the "guard" hypothesis of innate immunity."xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m307321200"xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m307321200"xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/author | "Giglione C."xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/author | "Giglione C."xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/author | "Meinnel T."xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/author | "Meinnel T."xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/author | "Boisson B."xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/author | "Boisson B."xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/pages | "43418-43429"xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/pages | "43418-43429"xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/title | "Unexpected protein families including cell defense components feature in the N-myristoylome of a higher eukaryote."xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/title | "Unexpected protein families including cell defense components feature in the N-myristoylome of a higher eukaryote."xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12912986 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12912986 |
| http://purl.uniprot.org/citations/12912986 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12912986 |
| http://purl.uniprot.org/citations/12912986 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12912986 |
| http://purl.uniprot.org/citations/12912986 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12912986 |