| http://purl.uniprot.org/citations/12924514 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12924514 | http://www.w3.org/2000/01/rdf-schema#comment | "Exposure in swine confinement facilities induces airway inflammation in healthy subjects. The aim of the present study was to elucidate the role of nuclear factor (NF)-kappaB in the inflammatory response induced by organic dust. A human lung epithelial carcinoma cell line (A549) was transfected with reporter genes of the human IL-6 promoter or the NF-kappaB binding site fused to the luciferase reporter gene and stimulated with dust from a swine confinement building. Cytokine release in cell culture supernatants and luciferase activity was measured. The dust-induced the activities of the IL-6 promoter reporter gene and the NF-kappaB reporter gene in parallel with an increase in IL-6 and IL-8 release. The addition of pyrrolidinedithiocarbamate, a chemical NF-kappaB blocking agent, inhibited IL-6 and IL-8 secretion as well as the NF-kappaB reporter gene activity. Increasing the amount of IkappaB alpha led to inhibition of organic dust-induced IL-6 promoter and NF-kappaB reporter gene activities. Fluticasone inhibited the organic dust-induced NF-kappaB activation and IL-6 and IL-8 secretion. Finally, swine dust incubation of A549 cells resulted in a NF-kappaB DNA binding, which is composed of the NF-kappaB1 and RelA proteins. In conclusion, by interference at various levels we have shown that NF-kappaB plays a key role in the inflammatory response to organic dust."xsd:string |
| http://purl.uniprot.org/citations/12924514 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0954-6111(03)00111-2"xsd:string |
| http://purl.uniprot.org/citations/12924514 | http://purl.uniprot.org/core/author | "Larsson K."xsd:string |
| http://purl.uniprot.org/citations/12924514 | http://purl.uniprot.org/core/author | "Okret S."xsd:string |
| http://purl.uniprot.org/citations/12924514 | http://purl.uniprot.org/core/author | "Liden J."xsd:string |
| http://purl.uniprot.org/citations/12924514 | http://purl.uniprot.org/core/author | "Palmberg L."xsd:string |
| http://purl.uniprot.org/citations/12924514 | http://purl.uniprot.org/core/author | "Ek A."xsd:string |
| http://purl.uniprot.org/citations/12924514 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12924514 | http://purl.uniprot.org/core/name | "Respir Med"xsd:string |
| http://purl.uniprot.org/citations/12924514 | http://purl.uniprot.org/core/pages | "882-892"xsd:string |
| http://purl.uniprot.org/citations/12924514 | http://purl.uniprot.org/core/title | "Organic dust activates NF-kappaB in lung epithelial cells."xsd:string |
| http://purl.uniprot.org/citations/12924514 | http://purl.uniprot.org/core/volume | "97"xsd:string |
| http://purl.uniprot.org/citations/12924514 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12924514 |
| http://purl.uniprot.org/citations/12924514 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12924514 |
| http://purl.uniprot.org/uniprot/Q04206#attribution-E4B379064A21BC91DA282BC22BBBB01D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12924514 |