| http://purl.uniprot.org/citations/12927542 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12927542 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12927542 | http://www.w3.org/2000/01/rdf-schema#comment | "beta1,4-Galactosyltransferase-I (beta4Gal-T1) undergoes critical conformational changes upon substrate binding from an open conformation (conf-I) to the closed conformation (conf-II). This change involves two flexible loops: the small (residues 313-316) and the long loop (residues 345-365). Upon substrate binding, Trp314 in the small flexible loop moves towards the catalytic pocket and interacts with the donor and the acceptor substrates. For a better understanding of the role played by Trp314 in the conformational changes of beta4Gal-T1, we mutated it to Ala and carried out substrate-binding, proteolytic and crystallographic studies. The W314A mutation reduces the enzymatic activity, binding to substrates and to the modifier protein, alpha-lactalbumin (LA), by over 99%. The limited proteolysis with Glu-C or Lys-C proteases shows differences in the rate of cleavage of the long loop of the wild-type and mutant W314A, indicating conformational differences in the region between the two proteins. Without substrate, the mutant crystallizes in a conformation (conf-I') (1.9A resolution crystal structure), that is not identical with, but close to an open conformation (conf-I), whereas its complex with the substrates and alpha-lactalbumin, crystallizes in a conformation (2.3A resolution crystal structure) that is identical with the closed conformation (conf-II). This study shows the crucial role Trp314 plays in the conformational state of the long loop, in the binding of substrates and in the catalytic mechanism of the enzyme."xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0022-2836(03)00790-3"xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0022-2836(03)00790-3"xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/author | "Ramakrishnan B."xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/author | "Ramakrishnan B."xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/author | "Boeggeman E."xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/author | "Boeggeman E."xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/author | "Qasba P.K."xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/author | "Qasba P.K."xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/author | "Ramasamy V."xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/author | "Ramasamy V."xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/pages | "1065-1076"xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/pages | "1065-1076"xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/title | "The role of tryptophan 314 in the conformational changes of beta1,4-galactosyltransferase-I."xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/title | "The role of tryptophan 314 in the conformational changes of beta1,4-galactosyltransferase-I."xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/volume | "331"xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://purl.uniprot.org/core/volume | "331"xsd:string |
| http://purl.uniprot.org/citations/12927542 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12927542 |
| http://purl.uniprot.org/citations/12927542 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12927542 |