| http://purl.uniprot.org/citations/12933801 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12933801 | http://www.w3.org/2000/01/rdf-schema#comment | "Surfactant protein C (SP-C) is a lung-specific protein that is synthesized as a 21-kDa integral membrane propeptide (pro-SP-C) and proteolytically processed to a 3.7-kDa secretory product. Previous studies have shown that palmitoylation of pro-SP-C is dependent on two N-terminal juxtamembrane positively charged residues. We hypothesized that these residues influence modification of pro-SP-C by directing transmembrane orientation. Double substitution mutation of these juxtaposed residues from positive to neutral charged species resulted in complete reversal of transmembrane orientation of pro-SP-C and total abrogation of post-translational processing. Mutation of a single residue resulted in mixed orientation. Protein trafficking studies in A549 cells showed that while the double mutant was retained in the endoplasmic reticulum, single mutants produced a mixed pattern of both endoplasmic reticulum (double mutant-like) and vesicular (wild type-like) expression. Our study demonstrates the crucial role juxtamembrane positively charged residues play in establishing membrane topology and their influence on the trafficking and processing of pro-SP-C. Moreover this study provides a likely precedent for a mechanism in disorders associated with mutations in the membrane-flanking region of integral membrane proteins."xsd:string |
| http://purl.uniprot.org/citations/12933801 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m308210200"xsd:string |
| http://purl.uniprot.org/citations/12933801 | http://purl.uniprot.org/core/author | "Beers M.F."xsd:string |
| http://purl.uniprot.org/citations/12933801 | http://purl.uniprot.org/core/author | "Mulugeta S."xsd:string |
| http://purl.uniprot.org/citations/12933801 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12933801 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/12933801 | http://purl.uniprot.org/core/pages | "47979-47986"xsd:string |
| http://purl.uniprot.org/citations/12933801 | http://purl.uniprot.org/core/title | "Processing of surfactant protein C requires a type II transmembrane topology directed by juxtamembrane positively charged residues."xsd:string |
| http://purl.uniprot.org/citations/12933801 | http://purl.uniprot.org/core/volume | "278"xsd:string |
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