| http://purl.uniprot.org/citations/12941800 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12941800 | http://www.w3.org/2000/01/rdf-schema#comment | "SR31747A is a sigma ligand with potent antiproliferative activity against tumor cells and for which three binding proteins have been identified to date: (a) SRBP-1 (also called sigma 1); (b) HIS; and (c) sigma 2. In this study, we characterized an additional SR31747A binding site, i.e., SRBP-2 (SR31747A-binding protein 2). Using an in silico screening approach, we identified this novel sequence, which exhibits 41% homology with HSI. The 1142-bp cDNA was found to encode a 206 amino acid protein not related to SRBP-1. Northern blot analysis of SRBP-2 mRNA expression revealed a single 1.1-kb transcript that was widely expressed in organs; the liver was particularly enriched, and the brain showed the lowest abundance. A murine homologue that exhibited a similar expression pattern was also characterized. Subcellular localization analysis using specific polyclonal antibodies revealed that SRBP-2 had the same nuclear membrane and endoplasmic reticulum localization as other members of the SR31747A-binding protein family. Considering SRBP-2-binding properties, pharmacological analysis clearly highlighted that SRBP-2 was distinct from sigma 2. Scatchard plot analysis revealed K(d) values of 10 and 3 nM for SR31747A and Tamoxifen, respectively. In contrast with HSI, the protein also did not exhibit detectable isomerase activity. When analyzing SRBP-2 expression in human breast cancer biopsies, we obtained evidence that SRBP-2 expression, together with SRBP-1 and HSI, may be of interest as a prognostic marker. These findings demonstrated that SRBP-2 represents an additional molecular target for SR31747A, which could help to understand the immunosuppressive and antiproliferative effects of the molecule."xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/author | "Loison G."xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/author | "Mondesert G."xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/author | "Dupuy P.H."xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/author | "Carayon P."xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/author | "Carriere D."xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/author | "Casellas P."xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/author | "Galiegue S."xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/author | "Combes T."xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/author | "Vidal H."xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/author | "Bribes E."xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/author | "Kramar A."xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/author | "Simony-Lafontaine J."xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/name | "Cancer Res"xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/pages | "4809-4818"xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/title | "Identification and pharmacological characterization of SRBP-2: a novel SR31747A-binding protein."xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://purl.uniprot.org/core/volume | "63"xsd:string |
| http://purl.uniprot.org/citations/12941800 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12941800 |
| http://purl.uniprot.org/citations/12941800 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12941800 |
| http://purl.uniprot.org/uniprot/Q12772#attribution-1DEB4D10573845E376ACF4C4BC86D2D1 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/12941800 |