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http://purl.uniprot.org/citations/12943527http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12943527http://www.w3.org/2000/01/rdf-schema#comment"

Objective

We have reported that endothelin-1 (ET-1) activates p38 mitogen-activated protein (MAP) kinase through protein kinase C in osteoblast-like MC3T3-E1 cells, and that p38 MAP kinase plays a role in the ET-1-induced heat shock protein 27 (HSP27). Recently, we found that stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) is activated by ET-1 in these cells. In the present study, we have investigated the involvement of SAPK/JNK in ET-1-induced HSP27 in MC3T3-E1 cells.

Methods

The concentration of HSP27 in soluble extracts of the cells, the expression of mRNA for HSP27, and the phosphorylation of SAPK/JNK were determined by an enzyme immunoassay, Northern blot analysis, and Western blot analysis respectively.

Results

SP600125, a specific inhibitor of SAPK/JNK, markedly reduced ET-1-stimulated HSP27 accumulation. The inhibitory effect of SP600125 was dose dependent in the range between 1 and 50 microM. SP600125 reduced the ET-1-increased level of HSP27 mRNA. Calphostin C and Go 6976, inhibitors of protein kinase C, reduced the ET-1-induced phosphorylation of SAPK/JNK. 12-O-Tetradecanoylphorbol-13-acetate, a direct activator of protein kinase C, induced SAPK/JNK phosphorylation, which was suppressed by SP600125. A combination of SP600125 and p38 MAP kinase inhibitor such as SB203580 and PD169316 additively reduced the ET-1-stimulated accumulation of HSP27.

Conclusions

These results strongly suggest that JNK plays a part in ET-1-induced HSP27 in addition to p38 MAP kinase in osteoblasts."xsd:string
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http://purl.uniprot.org/citations/12943527http://purl.uniprot.org/core/author"Ito H."xsd:string
http://purl.uniprot.org/citations/12943527http://purl.uniprot.org/core/author"Kato K."xsd:string
http://purl.uniprot.org/citations/12943527http://purl.uniprot.org/core/author"Niwa M."xsd:string
http://purl.uniprot.org/citations/12943527http://purl.uniprot.org/core/author"Tokuda H."xsd:string
http://purl.uniprot.org/citations/12943527http://purl.uniprot.org/core/author"Oiso Y."xsd:string
http://purl.uniprot.org/citations/12943527http://purl.uniprot.org/core/author"Kozawa O."xsd:string
http://purl.uniprot.org/citations/12943527http://purl.uniprot.org/core/date"2003"xsd:gYear
http://purl.uniprot.org/citations/12943527http://purl.uniprot.org/core/name"Eur J Endocrinol"xsd:string
http://purl.uniprot.org/citations/12943527http://purl.uniprot.org/core/pages"239-245"xsd:string
http://purl.uniprot.org/citations/12943527http://purl.uniprot.org/core/title"Involvement of stress-activated protein kinase/c-Jun N-terminal kinase in endothelin-1-induced heat shock protein 27 in osteoblasts."xsd:string
http://purl.uniprot.org/citations/12943527http://purl.uniprot.org/core/volume"149"xsd:string
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