| http://purl.uniprot.org/citations/12944398 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12944398 | http://www.w3.org/2000/01/rdf-schema#comment | "alpha-Melanocyte-stimulating hormone (MSH) utilizes cAMP to trigger pigmentation of melanocytes via activation of melanocyte-restricted microphthalmia-associated transcription factor (M-MITF) expression. M-MITF is a melanocyte-restricted helix-loop-helix transcription factor capable of transactivating promoters for multiple genes whose products modulate pigmentation. Although M-MITF promoter activation by MSH is known to occur through a conserved cAMP-response element (CRE), it remains unclear how this CRE exhibits such exquisitely tissue-restricted responsiveness. Here we show that cAMP-mediated CRE-binding protein activation of the M-MITF promoter requires a second DNA element located approximately 100 bp upstream, a site that is bound and activated by SOX10. Mutations in the SOX10 transcription factor, like MITF, results in a disorder known as Waardenburg Syndrome. The cAMP response of the M-MITF promoter was analyzed in melanoma and neuroblastoma cells (which are neural crest-derived but lack both M-MITF and SOX10 expression). M-MITF promoter responsiveness to cAMP was found to depend upon SOX10, and reciprocally, SOX10 transactivation was dependent upon the CRE. Ectopic SOX10 expression, in cooperation with cAMP signaling, activated the M-MITF promoter function and the expression of measurable endogenous M-MITF transcripts in neuroblastoma cells. SOX10dom, a mutant allele, failed to cooperate with cAMP in neuroblastoma cells and attenuated the cAMP responsiveness of the M-MITF promoter in melanoma cells. These observations demonstrate a means whereby the ubiquitous cAMP signaling machinery is harnessed to produce a highly tissue-restricted transcriptional response by cooperating with architectural factors, in this case SOX10."xsd:string |
| http://purl.uniprot.org/citations/12944398 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m309036200"xsd:string |
| http://purl.uniprot.org/citations/12944398 | http://purl.uniprot.org/core/author | "Du J."xsd:string |
| http://purl.uniprot.org/citations/12944398 | http://purl.uniprot.org/core/author | "Davis I.J."xsd:string |
| http://purl.uniprot.org/citations/12944398 | http://purl.uniprot.org/core/author | "Wegner M."xsd:string |
| http://purl.uniprot.org/citations/12944398 | http://purl.uniprot.org/core/author | "Huber W.E."xsd:string |
| http://purl.uniprot.org/citations/12944398 | http://purl.uniprot.org/core/author | "Fisher D.E."xsd:string |
| http://purl.uniprot.org/citations/12944398 | http://purl.uniprot.org/core/author | "Widlund H.R."xsd:string |
| http://purl.uniprot.org/citations/12944398 | http://purl.uniprot.org/core/author | "Price E.R."xsd:string |
| http://purl.uniprot.org/citations/12944398 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12944398 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/12944398 | http://purl.uniprot.org/core/pages | "45224-45230"xsd:string |
| http://purl.uniprot.org/citations/12944398 | http://purl.uniprot.org/core/title | "A tissue-restricted cAMP transcriptional response: SOX10 modulates alpha-melanocyte-stimulating hormone-triggered expression of microphthalmia-associated transcription factor in melanocytes."xsd:string |
| http://purl.uniprot.org/citations/12944398 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12944398 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12944398 |
| http://purl.uniprot.org/citations/12944398 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/12944398 |
| http://purl.uniprot.org/uniprot/#_A0A087WXU1-mappedCitation-12944398 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12944398 |
| http://purl.uniprot.org/uniprot/#_A0A0H3W5T2-mappedCitation-12944398 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12944398 |
| http://purl.uniprot.org/uniprot/#_P56693-mappedCitation-12944398 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12944398 |
| http://purl.uniprot.org/uniprot/#_A0A8F9W6H4-mappedCitation-12944398 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12944398 |
| http://purl.uniprot.org/uniprot/#_A0A0A7NWS7-mappedCitation-12944398 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12944398 |
| http://purl.uniprot.org/uniprot/#_B4DNC7-mappedCitation-12944398 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12944398 |
| http://purl.uniprot.org/uniprot/#_A6XND7-mappedCitation-12944398 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12944398 |
| http://purl.uniprot.org/uniprot/#_A8K5K3-mappedCitation-12944398 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/12944398 |