http://purl.uniprot.org/citations/12951494 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/12951494 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/12951494 | http://www.w3.org/2000/01/rdf-schema#comment | "Novel antimicrobial peptides (AMP), designated Fa-AMP1 and Fa-AMP2, were purified from the seeds of buckwheat (Fagopyrum esculentum Moench.) by gel filtration on Sephadex G75, ion-exchange HPLC on SP COSMOGEL, and reverse-phase HPLC. They were basic peptides having isoelectric points of over 10. Fa-AMP1 and Fa-AMP2 had molecular masses of 3,879 Da and 3,906 Da on MALDI-TOF MS analysis, and their extinction coefficients in 1% aqueous solutions at 280 nm were 42.8 and 38.9, respectively. Half of all amino acid residues of Fa-AMP1 and Fa-AMP2 were cysteine and glycine, and they had continuous sequences of cysteine and glycine. The concentrations of peptides required for 50% inhibition (IC50) of the growth of plant pathogenic fungi, and Gram-positive and -negative bacteria were 11 to 36 microg/ml. The structural and antimicrobial characteristics of Fa-AMPs indicated that they are a novel type of antimicrobial peptides belonging to a plant defensin family."xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.org/dc/terms/identifier | "doi:10.1271/bbb.67.1636"xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.org/dc/terms/identifier | "doi:10.1271/bbb.67.1636"xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/author | "Minami Y."xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/author | "Minami Y."xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/author | "Watanabe K."xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/author | "Watanabe K."xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/author | "Fujimura M."xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/author | "Fujimura M."xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/author | "Tadera K."xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/author | "Tadera K."xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/name | "Biosci. Biotechnol. Biochem."xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/name | "Biosci. Biotechnol. Biochem."xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/pages | "1636-1642"xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/pages | "1636-1642"xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/title | "Purification, characterization, and sequencing of a novel type of antimicrobial peptides, Fa-AMP1 and Fa-AMP2, from seeds of buckwheat (Fagopyrum esculentum Moench.)."xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/title | "Purification, characterization, and sequencing of a novel type of antimicrobial peptides, Fa-AMP1 and Fa-AMP2, from seeds of buckwheat (Fagopyrum esculentum Moench.)."xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/volume | "67"xsd:string |
http://purl.uniprot.org/citations/12951494 | http://purl.uniprot.org/core/volume | "67"xsd:string |
http://purl.uniprot.org/citations/12951494 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12951494 |
http://purl.uniprot.org/citations/12951494 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12951494 |