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http://purl.uniprot.org/citations/12963116http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12963116http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/12963116http://www.w3.org/2000/01/rdf-schema#comment"Macrocysts in Dictyostelium discoideum possess prototypic features of sexual reproduction and are useful for understanding the basic mechanisms of the reproductive process. Here, we randomly analyzed 1,071 gamete cDNAs, and then constructed a gamete-specific subtraction library, FC-IC. Nucleotide sequences of all 903 FC-IC clones were determined and clustered into 272 independent genes. Expression analysis based on real-time RT-PCR revealed 67 gamete-enriched genes, among which those involved in 'signal transduction' and 'multicellular organization' are prevalent. One of them, FC-IC0003, appeared also to be mating-type specific, and was named gmsA. RNAi-mediated silencing as well as disruption of gmsA reduced the cellular competency for sexual cell fusion, indicating the involvement of this gene in the sexual development of D. discoideum."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.org/dc/terms/identifier"doi:10.1016/s0925-4773(03)00136-9"xsd:string
http://purl.uniprot.org/citations/12963116http://purl.org/dc/terms/identifier"doi:10.1016/s0925-4773(03)00136-9"xsd:string
http://purl.uniprot.org/citations/12963116http://purl.org/dc/terms/identifier"doi:10.1016/S0925-4773(03)00136-9"xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Kohara Y."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Kohara Y."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Tanaka Y."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Tanaka Y."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Suzuki K."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Suzuki K."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Shimizu H."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Shimizu H."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Urushihara H."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Urushihara H."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Muramoto T."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Muramoto T."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Kohriki E."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Kohriki E."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Obara S."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/author"Obara S."xsd:string
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/date"2003"xsd:gYear
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/date"2003"xsd:gYear
http://purl.uniprot.org/citations/12963116http://purl.uniprot.org/core/name"Mech. Dev."xsd:string