| http://purl.uniprot.org/citations/12966087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12966087 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12966087 | http://www.w3.org/2000/01/rdf-schema#comment | "The structural maintenance of chromosomes (SMC) family of proteins play essential roles in genomic stability. SMC heterodimers are required for sister-chromatid cohesion (Cohesin: Smc1 & Smc3), chromatin condensation (Condensin: Smc2 & Smc4), and DNA repair (Smc5 & Smc6). The SMC heterodimers do not function alone and must associate with essential non-SMC subunits. To gain further insight into the essential and DNA repair roles of the Smc5-6 complex, we have purified fission yeast Smc5 and identified by mass spectrometry the co-precipitating proteins, Nse1 and Nse2. We show that both Nse1 and Nse2 interact with Smc5 in vivo, as part of the Smc5-6 complex. Nse1 and Nse2 are essential proteins and conserved from yeast to man. Loss of Nse1 and Nse2 function leads to strikingly similar terminal phenotypes to those observed for Smc5-6 inactivation. In addition, cells expressing hypomorphic alleles of Nse1 and Nse2 are, like Smc5-6 mutants, hypersensitive to DNA damage. Epistasis analysis suggests that like Smc5-6, Nse1, and Nse2 function together with Rhp51 in the homologous recombination repair of DNA double strand breaks. The results of this study strongly suggest that Nse1 and Nse2 are novel non-SMC subunits of the fission yeast Smc5-6 DNA repair complex."xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m308828200"xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m308828200"xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/author | "Yates J.R. III"xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/author | "Yates J.R. III"xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/author | "McDonald W.H."xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/author | "McDonald W.H."xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/author | "Boddy M.N."xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/author | "Boddy M.N."xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/author | "Pavlova Y."xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/author | "Pavlova Y."xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/pages | "45460-45467"xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/pages | "45460-45467"xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/title | "Novel essential DNA repair proteins Nse1 and Nse2 are subunits of the fission yeast Smc5-Smc6 complex."xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/title | "Novel essential DNA repair proteins Nse1 and Nse2 are subunits of the fission yeast Smc5-Smc6 complex."xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/12966087 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12966087 |
| http://purl.uniprot.org/citations/12966087 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/12966087 |