| http://purl.uniprot.org/citations/12970194 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12970194 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12970194 | http://www.w3.org/2000/01/rdf-schema#comment | "Iron regulatory protein 1 (IRP1) is regulated through the assembly/disassembly of a [4Fe-4S] cluster, which interconverts IRP1 with cytosolic aconitase. A genetic screen to isolate Saccharomyces cerevisiae strains bearing mutations in genes required for the conversion of IRP1 to c-aconitase led to the identification of a previously uncharacterized, essential gene, which we call CFD1 (cytosolic Fe-S cluster deficient). CFD1 encodes a highly conserved, putative P-loop ATPase. A non-lethal mutation of CFD1 (cfd1-1) reduced c-aconitase specific activity in IRP1-transformed yeast by >90%, although IRP1 in these cells could be readily converted to c-aconitase in vitro upon incubation with iron alone. IRP1-transformed cfd1-1 yeast lacked EPR-detectable Fe-S clusters in c-aconitase, pointing to a defect in Fe-S cluster assembly. The specific activity of another cytosolic Fe-S protein, Leu1p, was also inhibited by >90% in cfd1-1 yeast, whereas activity of mitochondrial Fe-S proteins was not inhibited. Consistent with a cytosolic site of activity, Cfd1p was localized in the cytoplasm. To our knowledge, Cfd1p is the first cytoplasmic Fe-S cluster assembly factor described in eukaryotes."xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/cdg455"xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/cdg455"xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/author | "Roy A."xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/author | "Roy A."xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/author | "Walden W.E."xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/author | "Walden W.E."xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/author | "Antholine W."xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/author | "Antholine W."xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/author | "Nicholson T."xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/author | "Nicholson T."xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/author | "Solodovnikova N."xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/author | "Solodovnikova N."xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/pages | "4826-4835"xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/pages | "4826-4835"xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/title | "A novel eukaryotic factor for cytosolic Fe-S cluster assembly."xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/title | "A novel eukaryotic factor for cytosolic Fe-S cluster assembly."xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/volume | "22"xsd:string |
| http://purl.uniprot.org/citations/12970194 | http://purl.uniprot.org/core/volume | "22"xsd:string |