| http://purl.uniprot.org/citations/12972652 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12972652 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/12972652 | http://www.w3.org/2000/01/rdf-schema#comment | "Protein serine/threonine phosphatase 5 (PP5) plays an important role in signal transduction in animal cells, but in plants, knowledge about PP5 is scarce. Here, we describe the isolation of a full-length cDNA encoding tomato (Lycopersicon esculentum) PP5 (LePP5) and its expression in Escherichia coli. Biochemical characterization showed that recombinant LePP5 has a low intrinsic protein phosphatase activity. This activity was increased 6-to 10-fold by either removal of the N-terminal tetratricopeptide repeat domain or by addition of fatty acids, indicating that biochemical features specific for PP5 homologs from other species are conserved in tomato. The single-copy LePP5 gene was cloned and shown to encode two mRNA species that arise by alternative pre-mRNA splicing. Similarly, Arabidopsis was found to express two PP5 transcripts, suggesting that alternative splicing of PP5 pre-mRNA is not specific for tomato. Alternative splicing results in a larger transcript containing an additional exon encoding two putative transmembrane domains and, hence, in a larger PP5 isoform. Subcellular fractionation studies on tomato protein lysates indicated that the majority of the 55-kD LePP5 isoform is soluble, whereas the 62-kD isoform is an integral membrane protein. Production of yellow fluorescent protein-PP5 chimeras in plant cells indicated that the 55-kD isoform is localized in both the nucleus and the cytoplasm, whereas the 62-kD isoform is targeted to the endoplasmic reticulum, including the nuclear envelope. Our findings show that alternative splicing generates two LePP5 isoforms with a different subcellular localization."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.org/dc/terms/identifier | "doi:10.1104/pp.103.026617"xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.org/dc/terms/identifier | "doi:10.1104/pp.103.026617"xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/author | "Cornelissen B.J.C."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/author | "Cornelissen B.J.C."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/author | "de la Fuente van Bentem S."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/author | "de la Fuente van Bentem S."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/author | "Haring M.A."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/author | "Haring M.A."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/author | "Vermeer J.E.M."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/author | "Vermeer J.E.M."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/author | "Gadella T.W.J. Jr."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/author | "Gadella T.W.J. Jr."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/author | "Vossen J.H."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/author | "Vossen J.H."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/author | "de Vroomen M.J."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/author | "de Vroomen M.J."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/name | "Plant Physiol."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/name | "Plant Physiol."xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/pages | "702-712"xsd:string |
| http://purl.uniprot.org/citations/12972652 | http://purl.uniprot.org/core/pages | "702-712"xsd:string |