| http://purl.uniprot.org/citations/13129928 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/13129928 | http://www.w3.org/2000/01/rdf-schema#comment | "Surfactant protein B (SP-B) is a critical component of pulmonary surfactant, and a deficiency of active SP-B results in fatal respiratory failure. SP-B is synthesized by type-II pneumocytes as a 42-kDa propeptide (proSP-B), which is posttranslationally processed to an 8-kDa surface-active protein. Napsin A is an aspartic protease expressed in type-II pneumocytes. To characterize the role of napsin A in the processing of proSP-B, we colocalized napsin A and precursors of SP-B as well as SP-B in the Golgi complex, multivesicular, composite, and lamellar bodies of type-II pneumocytes in human lungs using immunogold labeling. Furthermore, we measured aspartic protease activity in isolated lamellar bodies as well as isolated human type-II pneumocytes and studied the cleavage of proSP-B by napsin A and isolated lamellar bodies in vitro. Both, napsin A and isolated lamellar bodies cleaved proSP-B and generated three identical processing products. Processing of proSP-B by isolated lamellar bodies was completely inhibited by an aspartic protease inhibitor. Sequence analysis of proSP-B processing products revealed several cleavage sites in the N- and C-terminal propeptides as well as one in the mature peptide. Two of the four processing products generated in vitro were also detected in type-II pneumocytes. In conclusion, our results show that napsin A is involved in the N- and C-terminal processing of proSP-B in type-II pneumocytes."xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m306844200"xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/author | "Richter J."xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/author | "Brasch F."xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/author | "Johnen G."xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/author | "Ochs M."xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/author | "Giller T."xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/author | "Kapp N."xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/author | "Muller K.M."xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/author | "Hawgood S."xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/author | "Buhling F."xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/author | "Derrick M."xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/author | "Schauer-Vukasinovic V."xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/author | "Kahne T."xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/author | "Guttentag S."xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/pages | "49006-49014"xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/title | "Involvement of napsin A in the C- and N-terminal processing of surfactant protein B in type-II pneumocytes of the human lung."xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/13129928 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/13129928 |
| http://purl.uniprot.org/citations/13129928 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/13129928 |
| http://purl.uniprot.org/uniprot/#_A0SEC1-mappedCitation-13129928 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/13129928 |
| http://purl.uniprot.org/uniprot/#_B2RC55-mappedCitation-13129928 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/13129928 |