| http://purl.uniprot.org/citations/1316937 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1316937 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1316937 | http://www.w3.org/2000/01/rdf-schema#comment | "The nucleotide sequence of the proBA operon from a proline-hyperproducing mutant of Serratia marcescens was determined. Two base substitutions were found: one in the proB structural gene, coding for gamma-glutamyl kinase (GK), and a second one in the promoter region of the operon. The former base substitution led to a change of the predicted amino acid at position 117 from an alanine to a valine in GK. This mutation rendered GK 700-fold less sensitive to proline-mediated feedback inhibition than the wild-type enzyme. The other base substitution, a transversion from a G-C to an A-T, was located in the spacer region between the '-35' and '-10' sequences of the promoter, and it increased the transcriptional activity of this operon fourfold. Both these two base substitutions, which were acquired at the step of selecting mutants resistant to a toxic proline analogue, 3,4-dehydroproline, confer upon cells a high proline productivity and an increased osmotolerance."xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.org/dc/terms/identifier | "doi:10.1099/00221287-138-4-693"xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.org/dc/terms/identifier | "doi:10.1099/00221287-138-4-693"xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/author | "Imai Y."xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/author | "Imai Y."xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/author | "Suzuki S."xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/author | "Suzuki S."xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/author | "Komatsubara S."xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/author | "Komatsubara S."xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/author | "Omori K."xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/author | "Omori K."xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/name | "J. Gen. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/name | "J. Gen. Microbiol."xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/pages | "693-699"xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/pages | "693-699"xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/title | "Analysis of the mutant proBA operon from a proline-producing strain of Serratia marcescens."xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/title | "Analysis of the mutant proBA operon from a proline-producing strain of Serratia marcescens."xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/volume | "138"xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://purl.uniprot.org/core/volume | "138"xsd:string |
| http://purl.uniprot.org/citations/1316937 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1316937 |
| http://purl.uniprot.org/citations/1316937 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1316937 |