| http://purl.uniprot.org/citations/1322500 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1322500 | http://www.w3.org/2000/01/rdf-schema#comment | "The normal cellular homologue of the acutely transforming oncogene v-raf is c-raf-1, which encodes a serine/threonine protein kinase that is activated by many extracellular stimuli. The physiological substrates of the protein c-Raf-1 are unknown. The mitogen-activated protein (MAP) kinases Erk1 and 2 are also activated by mitogens through phosphorylation of Erk tyrosine and threonine residues catalysed by a protein kinase of relative molecular mass 50,000, MAP kinase-kinase (MAPK-K). Here we report that MAPK-K as well as Erk1 and 2 are constitutively active in v-raf-transformed cells. MAPK-K partially purified from v-raf-transformed cells or from mitogen-treated cells can be deactivated by phosphatase 2A. c-Raf-1 purified after mitogen stimulation can reactivate the phosphatase 2A-inactivated MAPK-K over 30-fold in vitro. c-Raf-1 reactivation of MAPK-K coincides with the selective phosphorylation at serine/threonine residues of a polypeptide with M(r) 50,000 which coelutes precisely on cation-exchange chromatography with the MAPK-K activatable by c-Raf-1. These results indicate that c-Raf-1 is an immediate upstream activator of MAPK-K in vivo. To our knowledge, MAPK-K is the first physiological substrate of the c-raf-1 protooncogene product to be identified."xsd:string |
| http://purl.uniprot.org/citations/1322500 | http://purl.org/dc/terms/identifier | "doi:10.1038/358417a0"xsd:string |
| http://purl.uniprot.org/citations/1322500 | http://purl.uniprot.org/core/author | "Rapp U.R."xsd:string |
| http://purl.uniprot.org/citations/1322500 | http://purl.uniprot.org/core/author | "Avruch J."xsd:string |
| http://purl.uniprot.org/citations/1322500 | http://purl.uniprot.org/core/author | "Kyriakis J.M."xsd:string |
| http://purl.uniprot.org/citations/1322500 | http://purl.uniprot.org/core/author | "Zhang X.F."xsd:string |
| http://purl.uniprot.org/citations/1322500 | http://purl.uniprot.org/core/author | "Banerjee P."xsd:string |
| http://purl.uniprot.org/citations/1322500 | http://purl.uniprot.org/core/author | "Brautigan D.L."xsd:string |
| http://purl.uniprot.org/citations/1322500 | http://purl.uniprot.org/core/author | "App H."xsd:string |
| http://purl.uniprot.org/citations/1322500 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1322500 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/1322500 | http://purl.uniprot.org/core/pages | "417-421"xsd:string |
| http://purl.uniprot.org/citations/1322500 | http://purl.uniprot.org/core/title | "Raf-1 activates MAP kinase-kinase."xsd:string |
| http://purl.uniprot.org/citations/1322500 | http://purl.uniprot.org/core/volume | "358"xsd:string |
| http://purl.uniprot.org/citations/1322500 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1322500 |
| http://purl.uniprot.org/citations/1322500 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1322500 |
| http://purl.uniprot.org/uniprot/Q99N57#attribution-4F1E2D64057B5118090C133D5AB4B8FA | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/1322500 |
| http://purl.uniprot.org/uniprot/#_A0A0N4SV36-mappedCitation-1322500 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/1322500 |
| http://purl.uniprot.org/uniprot/#_A0A0N4SWH5-mappedCitation-1322500 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/1322500 |
| http://purl.uniprot.org/uniprot/#_A0A494B9N5-mappedCitation-1322500 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/1322500 |
| http://purl.uniprot.org/uniprot/#_F6TUC4-mappedCitation-1322500 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/1322500 |
| http://purl.uniprot.org/uniprot/#_Q8BLY7-mappedCitation-1322500 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/1322500 |
| http://purl.uniprot.org/uniprot/#_P13516-mappedCitation-1322500 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/1322500 |
| http://purl.uniprot.org/uniprot/#_Q3UXG5-mappedCitation-1322500 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/1322500 |