| http://purl.uniprot.org/citations/1322842 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1322842 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1322842 | http://www.w3.org/2000/01/rdf-schema#comment | "7-Chloro-4-nitro-benzofurazan selectively modifies one PPase Tyr residue per subunit and lowers the enzyme activity. Hydrolysis of the modified protein by trypsin and then by chymotrypsin produces the 82-89 peptide which possesses modified Tyr-89. Substrate analog (CaPPi) and the product of the enzyme reaction, MgPi, protect the enzyme against inactivation. Ions of metal-activators (Mg2+, Zn2+) exert no influence on the inactivation rate. On the contrary, the Ca(2+)-inhibitor of the enzyme accelerates the reaction by binding to the high-affinity site, and effectively decreases it when Ca2+ binds to both sites. Mg2+ competes with Ca2+ for one binding site, which is the low affinity site for Mg2+ and the high-affinity site for Ca2+. The Ca2+ saturation of the high-affinity site decreases the pK2 of Tyr-89, probably due to direct coordination between Tyr and Ca2+. The observed properties of Tyr-89 modification enable us to propose that Tyr-89 serves as a proton donor for phosphate releasing during enzymatic hydrolysis of pyrophosphate. The Ca2+ inhibitory effect on the enzyme activity may be due to the existence of a Tyr-89 bond in the Ca2+ pyrophosphatase complex."xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.org/dc/terms/identifier | "doi:10.1016/0014-5793(92)81051-m"xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.org/dc/terms/identifier | "doi:10.1016/0014-5793(92)81051-m"xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/author | "Avaeva S.M."xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/author | "Avaeva S.M."xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/author | "Raznikov A.V."xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/author | "Raznikov A.V."xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/author | "Sklyankina V.A."xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/author | "Sklyankina V.A."xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/name | "FEBS Lett."xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/name | "FEBS Lett."xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/pages | "62-64"xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/pages | "62-64"xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/title | "Tyrosine-89 is important for enzymatic activity of S. cerevisiae inorganic pyrophosphatase."xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/title | "Tyrosine-89 is important for enzymatic activity of S. cerevisiae inorganic pyrophosphatase."xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/volume | "308"xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://purl.uniprot.org/core/volume | "308"xsd:string |
| http://purl.uniprot.org/citations/1322842 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1322842 |
| http://purl.uniprot.org/citations/1322842 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1322842 |
| http://purl.uniprot.org/citations/1322842 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1322842 |
| http://purl.uniprot.org/citations/1322842 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1322842 |