| http://purl.uniprot.org/citations/1329945 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1329945 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1329945 | http://www.w3.org/2000/01/rdf-schema#comment | "It has been shown previously that Escherichia coli contains three fumarase genes designated fumA, fumB, and fumC. The gene products fumarases A, B, and C have been divided into two classes. Class I contains fumarases A and B, which have amino acid sequences that are 90% identical to each other, but have almost no similarity to the sequence of porcine fumarase. Class II contains fumarase C and porcine fumarase, which have amino acid sequences 60% identical to each other [Woods, S.A., Schwartzbach, S.D., & Guest, J.R. (1988) Biochim. Biophys. Acta 954, 14-26]. In this work it is shown that purified fumarase A contains a [4Fe-4S] cluster. This conclusion is based on the following observations. Fumarase A contains 4 Fe and 4 S2-per mole of protein monomer. (The mobility of fumarase A in native polyacrylamide gel electrophoresis and the elution volume on a gel permeation column indicate that it is a homodimer.) Its visible and circular dichroism spectra are characteristic of proteins containing an Fe-S cluster. Fumarase A can be reduced to an EPR active-state exhibiting a spectrum consisting of a rhombic spectrum at high fields (g-values = 2.03, 1.94, and 1.88) and a broad peak at g = 5.4. Upon addition of substrate, the high field signal shifts upfield (g-values = 2.035, 1.92, and 1.815) and increases in total spins by 8-fold, while the g = 5.4 signal disappears.(ABSTRACT TRUNCATED AT 250 WORDS)"xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00157a022"xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi00157a022"xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/author | "Guest J.R."xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/author | "Guest J.R."xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/author | "Flint D.H."xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/author | "Flint D.H."xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/author | "Emptage M.H."xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/author | "Emptage M.H."xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/pages | "10331-10337"xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/pages | "10331-10337"xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/title | "Fumarase a from Escherichia coli: purification and characterization as an iron-sulfur cluster containing enzyme."xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/title | "Fumarase a from Escherichia coli: purification and characterization as an iron-sulfur cluster containing enzyme."xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/volume | "31"xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://purl.uniprot.org/core/volume | "31"xsd:string |
| http://purl.uniprot.org/citations/1329945 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1329945 |
| http://purl.uniprot.org/citations/1329945 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1329945 |
| http://purl.uniprot.org/citations/1329945 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1329945 |
| http://purl.uniprot.org/citations/1329945 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1329945 |