| http://purl.uniprot.org/citations/1350248 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1350248 | http://www.w3.org/2000/01/rdf-schema#comment | "It has been reported that interleukin 6 (IL-6) acts on hemopoietic stem cells in synergism with interleukin 3 (IL-3), but it has not yet been clarified whether IL-6 acts directly on the stem cells or not. To investigate the mechanism of the synergism between IL-3 and IL-6, we sorted hemopoietic stem cells from untreated murine bone marrow cells using a two-laser fluorescence-activated cell sorter (FACS). Cells negative for the lymphohemopoietic lineage (lineage-negative, Lin-), with a high affinity to wheat germ agglutinin (WGA+), and showing a low expression of Thy-1 antigen (Thy-1low) were sorted and analyzed by in vitro and in vivo colony formation. This fraction was 0.4% of the total mononuclear bone marrow cells. Approximately 25% of these Lin-WGA+Thy-1low cells showed in vitro colony formation, whereas approximately 1% of them formed day-8 and day-12 spleen colonies. Thus, it appears that the Lin-WGA+Thy-1low cells were a highly enriched stem cell population. By FACS clone sorting, single cells were isolated from the enriched stem cell fraction and cultured in semisolid or liquid culture systems. Addition of IL-6 to methylcellulose medium containing IL-3 did not significantly increase the number of colonies. It is thus suggested that the target cells of IL-3 and IL-6 are the same as those of IL-3. The secondary colony-forming ability of primary colonies that developed in the presence of IL-6 and IL-3 was higher than that of colonies formed in the presence of IL-3 alone. In correspondence with this finding, the numbers of myeloid colonies and spleen colony-forming units (CFU-S) were increased by the incubation of these sorted cells for 7 days with IL-6 and IL-3 when compared with the effect of IL-3 alone. Therefore, it is concluded that IL-6 acts directly on hemopoietic stem cells to enhance their proliferation."xsd:string |
| http://purl.uniprot.org/citations/1350248 | http://purl.uniprot.org/core/author | "Nakamura M."xsd:string |
| http://purl.uniprot.org/citations/1350248 | http://purl.uniprot.org/core/author | "Okada S."xsd:string |
| http://purl.uniprot.org/citations/1350248 | http://purl.uniprot.org/core/author | "Nakauchi H."xsd:string |
| http://purl.uniprot.org/citations/1350248 | http://purl.uniprot.org/core/author | "Miura Y."xsd:string |
| http://purl.uniprot.org/citations/1350248 | http://purl.uniprot.org/core/author | "Suda T."xsd:string |
| http://purl.uniprot.org/citations/1350248 | http://purl.uniprot.org/core/author | "Nagayoshi K."xsd:string |
| http://purl.uniprot.org/citations/1350248 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1350248 | http://purl.uniprot.org/core/name | "Exp Hematol"xsd:string |
| http://purl.uniprot.org/citations/1350248 | http://purl.uniprot.org/core/pages | "546-551"xsd:string |
| http://purl.uniprot.org/citations/1350248 | http://purl.uniprot.org/core/title | "Synergistic effect of IL-3 and IL-6 on highly enriched murine hemopoietic progenitors."xsd:string |
| http://purl.uniprot.org/citations/1350248 | http://purl.uniprot.org/core/volume | "20"xsd:string |
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| http://purl.uniprot.org/uniprot/P01586#attribution-EC8913908305A87C3363F3EF9D2AD880 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/1350248 |
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