| http://purl.uniprot.org/citations/1367792 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1367792 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1367792 | http://www.w3.org/2000/01/rdf-schema#comment | "The major inhibitor of trypsin in seeds of Prosopsis juliflora was purified by precipitation with ammonium sulphate, ion-exchange column chromatography on DEAE- and CM-Sepharose and preparative reverse phase HPLC on a Vydac C-18 column. The protein inhibited trypsin in the stoichiometric ratio of 1:1, but had only weak activity against chymotrypsin and did not inhibit human salivary or porcine pancreatic alpha-amylases. SDS-PAGE indicated that the inhibitor has a Mr of ca 20,000, and IEF-PAGE showed that the pI is 8.8. The complete amino acid sequence was determined by automatic degradation, and by DABITC/PITC microsequence analysis of peptides obtained from enzyme digestions of the reduced and S-carboxymethylated protein with trypsin, chymotrypsin, elastase, the Glu-specific protease from S. aureus and the Lys-specific protease from Lysobacter enzymogenes. The inhibitor consisted of two polypeptide chains, of 137 residues (alpha chain) and 38 residues (beta chain) linked together by a single disulphide bond. The amino acid sequence of the protein exhibited homology with a number of Kunitz proteinase inhibitors from other legume seeds, the bifunctional subtilisin/alpha-amylase inhibitors from cereals and the taste-modifying protein miraculin."xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0031-9422(00)98207-4"xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0031-9422(00)98207-4"xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/author | "Shewry P.R."xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/author | "Shewry P.R."xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/author | "Richardson M."xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/author | "Richardson M."xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/author | "Blanco-Labra A."xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/author | "Blanco-Labra A."xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/author | "Carvalho M.M."xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/author | "Carvalho M.M."xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/author | "Negreiros A.N."xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/author | "Negreiros A.N."xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/author | "Xavier Filho J."xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/author | "Xavier Filho J."xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/date | "1991"xsd:gYear |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/name | "Phytochemistry"xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/name | "Phytochemistry"xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/pages | "2829-2833"xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/pages | "2829-2833"xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/title | "The complete amino acid sequence of the major Kunitz trypsin inhibitor from the seeds of Prosopsis juliflora."xsd:string |
| http://purl.uniprot.org/citations/1367792 | http://purl.uniprot.org/core/title | "The complete amino acid sequence of the major Kunitz trypsin inhibitor from the seeds of Prosopsis juliflora."xsd:string |