| http://purl.uniprot.org/citations/1368580 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1368580 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1368580 | http://www.w3.org/2000/01/rdf-schema#comment | "Aminopeptidase T (AP-T) is a metallo-dependent dimeric enzyme of Thermus aquaticus YT-1, an extremely thermophilic bacterium. We cloned the AP-T gene from T. aquaticus YT-1 into Escherichia coli using a synthetic oligonucleotide as a hybridization probe. The nucleotide sequence of the AP-T gene was found to encode 408 amino acid residues with GTG as a start codon. The molecular weight was calculated to be 44,820. The AP-T was overproduced in E. coli (about 5% of total soluble protein) when the start codon of the gene was changed from GTG to ATG, and the gene was downstream from the tac promoter. The AP-T expressed in E. coli was heat stable and easily purified by heat treatment (80 degrees C, 30 min). The N-terminal amino acid sequence of AP-T showed similarity with that of aminopeptidase II from Bacillus stearothermophilus."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.org/dc/terms/identifier | "doi:10.1271/bbb1961.54.2385"xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.org/dc/terms/identifier | "doi:10.1271/bbb1961.54.2385"xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Ohta T."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Ohta T."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Ono M."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Ono M."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Matsuzawa H."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Matsuzawa H."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Azuma N."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Azuma N."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Kaminogawa S."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Kaminogawa S."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Minagawa E."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Minagawa E."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Motoshima H."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Motoshima H."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Yamauchi K."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/author | "Yamauchi K."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/name | "Agric. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/1368580 | http://purl.uniprot.org/core/name | "Agric. Biol. Chem."xsd:string |