| http://purl.uniprot.org/citations/1374166 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1374166 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1374166 | http://www.w3.org/2000/01/rdf-schema#comment | "AIDS, caused by human immunodeficiency virus (HIV), is one of the world's most serious health problems, with current protocols being inadequate for either prevention or successful long-term treatment. In retroviruses such as HIV, the enzyme reverse transcriptase copies the single-stranded RNA genome into double-stranded DNA that is then integrated into the chromosomes of infected cells. Reverse transcriptase is the target of the most widely used treatments for AIDS, 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxyinosine (ddI), but resistant strains of HIV-1 arise in patients after a relatively short time. There are several nonnucleoside inhibitors of HIV-1 reverse transcriptase, but resistance to such agents also develops rapidly. We report here the structure at 7 A resolution of a ternary complex of the HIV-1 reverse transcriptase heterodimer, a monoclonal antibody Fab fragment, and a duplex DNA template-primer. The double-stranded DNA binds in a groove on the surface of the enzyme. The electron density near one end of the DNA matches well with the known structure of the HIV-1 reverse transcriptase RNase H domain. At the opposite end of the DNA, a mercurated derivative of UTP has been localized by difference Fourier methods, allowing tentative identification of the polymerase nucleoside triphosphate binding site. We also determined the structure of the reverse transcriptase/Fab complex in the absence of template-primer to compare the bound and free forms of the enzyme. The presence of DNA correlates with movement of protein electron density in the vicinity of the putative template-primer binding groove. These results have important implications for developing improved inhibitors of reverse transcriptase for the treatment of AIDS."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.org/dc/terms/identifier | "doi:10.1038/357085a0"xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.org/dc/terms/identifier | "doi:10.1038/357085a0"xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Ding J."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Ding J."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Clark P."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Clark P."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Lu X."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Lu X."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Williams R.L."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Williams R.L."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Zhang A."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Zhang A."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Arnold E."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Arnold E."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Hughes S.H."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Hughes S.H."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Hizi A."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Hizi A."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Clark A.D. Jr."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Clark A.D. Jr."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Ferris A.L."xsd:string |
| http://purl.uniprot.org/citations/1374166 | http://purl.uniprot.org/core/author | "Ferris A.L."xsd:string |