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http://purl.uniprot.org/citations/1396688http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/1396688http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/1396688http://www.w3.org/2000/01/rdf-schema#comment"A new member of the barley (1-->3)-beta-glucan glucanohydrolase family of enzymes has been purified from extracts of germinated grain and young seedlings by fractional precipitation with ammonium sulphate, ion-exchange chromatography, chromatofocussing and gel-filtration chromatography. The enzyme, which has been designated (1-->3)-beta-glucanase isoenzyme GIII, is a basic protein with an apparent molecular mass of 32 000 Da. Oligosaccharide products released by the enzyme during hydrolysis of the (1-->3)-beta-glucan, laminarin, indicate that the enzyme is an endohydrolase. A 2349-bp fragment of barley genomic DNA has been isolated and identified as the gene encoding the (1-->3)-beta-glucanase isoenzyme GIII. The open reading frame encoding the isoenzyme is interrupted by a single intron of 180 bp that splits a codon in the putative signal-peptide region. Northern-blot analyses with gene-specific probes indicate that the (1-->3)-beta-glucanase isoenzyme GIII mRNA accumulates in developing leaves; no mRNA transcripts were detected in the aleurone or scutellum of germinated grain, or in mature vegetative tissues. Although plant (1-->3)-beta-glucanases are generally classified as 'pathogenesis-related' proteins, the physiological function of the barley (1-->3)-beta-glucanase isoenzyme GIII is unclear."xsd:string
http://purl.uniprot.org/citations/1396688http://purl.org/dc/terms/identifier"doi:10.1111/j.1432-1033.1992.tb17266.x"xsd:string
http://purl.uniprot.org/citations/1396688http://purl.org/dc/terms/identifier"doi:10.1111/j.1432-1033.1992.tb17266.x"xsd:string
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/author"Wang J."xsd:string
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/author"Wang J."xsd:string
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/author"Xu P."xsd:string
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/author"Xu P."xsd:string
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/author"Fincher G.B."xsd:string
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/author"Fincher G.B."xsd:string
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/date"1992"xsd:gYear
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/date"1992"xsd:gYear
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/name"Eur. J. Biochem."xsd:string
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/name"Eur. J. Biochem."xsd:string
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/pages"103-109"xsd:string
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/pages"103-109"xsd:string
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/title"Purification, characterization and gene structure of (1-->3)-beta-glucanase isoenzyme GIII from barley (Hordeum vulgare)."xsd:string
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/title"Purification, characterization and gene structure of (1-->3)-beta-glucanase isoenzyme GIII from barley (Hordeum vulgare)."xsd:string
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/volume"209"xsd:string
http://purl.uniprot.org/citations/1396688http://purl.uniprot.org/core/volume"209"xsd:string
http://purl.uniprot.org/citations/1396688http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/1396688
http://purl.uniprot.org/citations/1396688http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/1396688
http://purl.uniprot.org/citations/1396688http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/1396688
http://purl.uniprot.org/citations/1396688http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/1396688