| http://purl.uniprot.org/citations/1396711 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1396711 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1396711 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/1396711 | http://www.w3.org/2000/01/rdf-schema#comment | "A novel enzyme, arylalkyl acylamidase, which shows a strict specificity for N-acetyl arylalkylamines, but not acetanilide derivatives, was purified from the culture broth of Pseudomonas putida Sc2. The purified enzyme appeared to be homogeneous, as judged by native and SDS/PAGE. The enzyme has a molecular mass of approximately 150 kDa and consists of four identical subunits. The purified enzyme catalyzed the hydrolysis of N-acetyl-2-phenylethylamine to 2-phenylethylamine and acetic acid at the rate of 6.25 mumol.min-1.mg-1 at 30 degrees C. It also catalyzed the hydrolysis of various N-acetyl arylalkylamines containing a benzene or indole ring, and acetic acid arylalkyl esters. The enzyme did not hydrolyze acetanilide, N-acetyl aliphatic amines, N-acetyl amino acids, N-acetyl amino sugars or acylthiocholine. The apparent Km for N-acetylbenzylamine, N-acetyl-2-phenylethylamine and N-acetyl-3-phenylpropylamine are 41 mM, 0.31 mM and 1.6 mM, respectively. The purified enzyme was sensitive to thiol reagents such as Ag2SO4, HgCl2 and p-chloromercuribenzoic acid, and its activity was enhanced by divalent metal ions such as Zn2+, Mg2+ and Mn2+."xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1992.tb17299.x"xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1432-1033.1992.tb17299.x"xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/author | "Shimizu S."xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/author | "Shimizu S."xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/author | "Yamada H."xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/author | "Yamada H."xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/author | "Ogawa J."xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/author | "Ogawa J."xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/author | "Chung M.C.-M."xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/author | "Chung M.C.-M."xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/name | "Eur. J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/pages | "375-382"xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/pages | "375-382"xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/title | "Purification and characterization of a novel enzyme, arylalkyl acylamidase, from Pseudomonas putida Sc2."xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/title | "Purification and characterization of a novel enzyme, arylalkyl acylamidase, from Pseudomonas putida Sc2."xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/volume | "209"xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://purl.uniprot.org/core/volume | "209"xsd:string |
| http://purl.uniprot.org/citations/1396711 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1396711 |