| http://purl.uniprot.org/citations/1448077 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1448077 | http://www.w3.org/2000/01/rdf-schema#comment | "We have identified a 50-nucleotide enhancer from the human erythropoietin gene 3'-flanking sequence which can mediate a sevenfold transcriptional induction in response to hypoxia when cloned 3' to a simian virus 40 promoter-chloramphenicol acetyltransferase reporter gene and transiently expressed in Hep3B cells. Nucleotides (nt) 1 to 33 of this sequence mediate sevenfold induction of reporter gene expression when present in two tandem copies compared with threefold induction when present in a single copy, suggesting that nt 34 to 50 bind a factor which amplifies the induction signal. DNase I footprinting demonstrated binding of a constitutive nuclear factor to nt 26 to 48. Mutagenesis studies revealed that nt 4 to 12 and 19 to 23 are essential for induction, as substitutions at either site eliminated hypoxia-induced expression. Electrophoretic mobility shift assays identified a nuclear factor which bound to a probe spanning nt 1 to 18 but not to a probe containing a mutation which eliminated enhancer function. Factor binding was induced by hypoxia, and its induction was sensitive to cycloheximide treatment. We have thus defined a functionally tripartite, 50-nt hypoxia-inducible enhancer which binds several nuclear factors, one of which is induced by hypoxia via de novo protein synthesis."xsd:string |
| http://purl.uniprot.org/citations/1448077 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.12.12.5447-5454.1992"xsd:string |
| http://purl.uniprot.org/citations/1448077 | http://purl.uniprot.org/core/author | "Wang G.L."xsd:string |
| http://purl.uniprot.org/citations/1448077 | http://purl.uniprot.org/core/author | "Semenza G.L."xsd:string |
| http://purl.uniprot.org/citations/1448077 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1448077 | http://purl.uniprot.org/core/name | "Mol Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/1448077 | http://purl.uniprot.org/core/pages | "5447-5454"xsd:string |
| http://purl.uniprot.org/citations/1448077 | http://purl.uniprot.org/core/title | "A nuclear factor induced by hypoxia via de novo protein synthesis binds to the human erythropoietin gene enhancer at a site required for transcriptional activation."xsd:string |
| http://purl.uniprot.org/citations/1448077 | http://purl.uniprot.org/core/volume | "12"xsd:string |
| http://purl.uniprot.org/citations/1448077 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/1448077 |
| http://purl.uniprot.org/citations/1448077 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/1448077 |
| http://purl.uniprot.org/uniprot/P27540#attribution-8FB87F631B001FA5658EA7B4067505A5 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/1448077 |
| http://purl.uniprot.org/uniprot/P27540#attribution-C1434ED539CBD56D54ED94DBEBE1E2D5 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/1448077 |
| http://purl.uniprot.org/uniprot/Q16665#attribution-8FB87F631B001FA5658EA7B4067505A5 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/1448077 |
| http://purl.uniprot.org/uniprot/Q16665#attribution-C1434ED539CBD56D54ED94DBEBE1E2D5 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/1448077 |