| http://purl.uniprot.org/citations/14508492 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14508492 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14508492 | http://www.w3.org/2000/01/rdf-schema#comment | "RNA interference (RNAi) regulates gene expression by the cleavage of messenger RNA, by mRNA degradation and by preventing protein synthesis. These effects are mediated by a ribonucleoprotein complex known as RISC (RNA-induced silencing complex). We have previously identified four Drosophila components (short interfering RNAs, Argonaute 2 (ref. 2), VIG and FXR) of a RISC enzyme that degrades specific mRNAs in response to a double-stranded-RNA trigger. Here we show that Tudor-SN (tudor staphylococcal nuclease)--a protein containing five staphylococcal/micrococcal nuclease domains and a tudor domain--is a component of the RISC enzyme in Caenorhabditis elegans, Drosophila and mammals. Although Tudor-SN contains non-canonical active-site sequences, we show that purified Tudor-SN exhibits nuclease activity similar to that of other staphylococcal nucleases. Notably, both purified Tudor-SN and RISC are inhibited by a specific competitive inhibitor of micrococcal nuclease. Tudor-SN is the first RISC subunit to be identified that contains a recognizable nuclease domain, and could therefore contribute to the RNA degradation observed in RNAi."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature01956"xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature01956"xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Hannon G.J."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Hannon G.J."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Plasterk R.H."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Plasterk R.H."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Ketting R.F."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Ketting R.F."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Caudy A.A."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Caudy A.A."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Bathoorn A.M."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Bathoorn A.M."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Denli A.M."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Denli A.M."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Hammond S.M."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Hammond S.M."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Myers M.M."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Myers M.M."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Silva J.M."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Silva J.M."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Tops B.B."xsd:string |
| http://purl.uniprot.org/citations/14508492 | http://purl.uniprot.org/core/author | "Tops B.B."xsd:string |