| http://purl.uniprot.org/citations/14530284 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14530284 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14530284 | http://www.w3.org/2000/01/rdf-schema#comment | "PIKfyve, a kinase that displays specificity for phosphatidylinositol (PtdIns), PtdIns 3-phosphate (3-P), and proteins, is important in multivesicular body/late endocytic function. Enzymatically inactive PIKfyve mutants elicit enormous dilation of late endocytic structures, suggesting a role for PIKfyve in endosome-to-trans-Golgi network (TGN) membrane retrieval. Here we report that p40, a Rab9 effector reported previously to bind Rab9-GTP and stimulate endosome-to-TGN transport, interacts with PIKfyve as determined by yeast two-hybrid assays, glutathione S-transferase (GST) pull-down assays, and co-immunoprecipitation in doubly transfected HEK293 cells. The interaction engages the PIKfyve chaperonin domain and four out of the six C-terminally positioned kelch repeats in p40. Differential centrifugation in a HEK293 cell line, stably expressing PIKfyveWT, showed the membrane-associated immunoreactive p40 co-sedimenting with PIKfyve in the high speed pellet (HSP) fraction. Remarkably, similar analysis in a HEK293 cell line stably expressing dominant-negative kinase-deficient PIKfyveK1831E demonstrated a marked depletion of p40 from the HSP fraction. GST-p40 failed to specifically associate with the PIKfyve lipid products PtdIns 5-P and PtdIns 3,5-P2 in a liposome binding assay but was found to be an in vitro substrate of the PIKfyve serine kinase activity. A band with the p40 electrophoretic mobility was found to react with a phosphoserine-specific antibody mainly in the PIKfyveWT-containing fractions obtained by density gradient sedimentation of total membranes from PIKfyveWT-expressing HEK293 cells. Together these results identify the Rab9 effector p40 as a PIKfyve partner and suggest that p40-PIKfyve interaction and the subsequent PIKfyve-catalyzed p40 phosphorylation anchor p40 to discrete membranes facilitating late endosome-to-TGN transport."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m307260200"xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m307260200"xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Finley R.L. Jr."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Finley R.L. Jr."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Shisheva A."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Shisheva A."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Ikonomov O.C."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Ikonomov O.C."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Sbrissa D."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Sbrissa D."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Deeb R."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Deeb R."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Fligger J."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Fligger J."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Mlak K."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Mlak K."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Soans A."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/author | "Soans A."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/14530284 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |