| http://purl.uniprot.org/citations/14531729 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14531729 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14531729 | http://www.w3.org/2000/01/rdf-schema#comment | "The gene dosage effect of the MC4-R (melanocortin 4 receptor) on obesity suggests that regulation of MC4-R expression and function is critically important to the central control of energy homoeostasis. In order to identify putative MC4-R regulatory proteins, we performed a yeast two-hybrid screen of a mouse brain cDNA library using the mouse MC4-R intracellular tail (residues 303-332) as bait. We report here on one positive clone that shares 63% amino acid identity with the C-terminal part of the mouse attractin gene product, a single-transmembrane-domain protein characterized as being required for agouti signalling through the melanocortin 1 receptor. We confirmed a direct interaction between this ALP (attractin-like protein) and the C-terminus of the mouse MC4-R by glutathione S-transferase pulldown experiments, and mapped the regions involved in this interaction using N- and C-terminal truncation constructs; residues 303-313 in MC4-R and residues 1280-1317 in ALP are required for binding. ALP is highly expressed in brain, but also in heart, lung, kidney and liver. Furthermore, co-localization analyses in mice showed co-expression of ALP in cells expressing MC4-R in a number of regions known to be important in the regulation of energy homoeostasis by melanocortins, such as the paraventricular nucleus of hypothalamus and the dorsal motor nucleus of the vagus."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20031241"xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20031241"xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Liu H."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Liu H."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Cone R.D."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Cone R.D."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Elmquist J.K."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Elmquist J.K."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Friedman J.M."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Friedman J.M."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Lee C.E."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Lee C.E."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Kishi T."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Kishi T."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Haqq A.M."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Haqq A.M."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Khong K."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Khong K."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Rene P."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/author | "Rene P."xsd:string |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14531729 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |