Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/14561752http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/14561752http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/14561752http://www.w3.org/2000/01/rdf-schema#comment"A common glycan alteration in transformed cells and human tumors is the highly elevated levels of N-linked beta(1,6)glycans caused by increased transcription of N-acetylglucosaminyltransferase V (GnT-V). Here, we define the involvement of GnT-V in modulation of homotypic cell-cell adhesion in human fibrosarcoma HT1080 and mouse NIH3T3 cells. Increased GnT-V expression resulted in a significant decrease in the rates of calcium-dependent cell-cell adhesion. Reduced cell-cell adhesion was blocked by function-blocking antibody against N-cadherin and abrogated by pre-treatment of cells with swainsonine, demonstrating the involvement of N-cadherin in the cell-cell adhesion and that changes in N-linked beta(1,6)glycan expression are responsible for the reduction in rates of adhesion, although this reduction could be mediated by the altered N-linked glycosylation of glycoproteins other than N-cadherin. Overexpression of GnT-V had no effect on the levels of cell surface expression of N-cadherin; however, it did cause a marked enhancement of both beta(1,6) branching and poly-N-acetyllactosamine expression on N-cadherin. GnT-V overexpression resulted in decreased N-cadherin clustering on the cell surface induced by anti-N-cadherin antibody and affected the outside-in signal transduction pathway of ERK mediated by N-cadherin. Overexpression of GnT-V sensitized stimulation of tyrosine phosphorylation of catenins by growth factors and expression of v-src, which is consistent with its reduction of cell-cell adhesion. In vitro, GnT-V-overexpressing cells showed increased motility concomitant with increased phosphorylation of catenins. Moreover, GnT-V-deficient embryo fibroblasts from GnT-V homozygous null mice (GnT-V(-/-)) express N-cadherin and showed significantly increased levels of N-cadherin-based cell-cell adhesion compared with those from GnT-V(+/-) mice. These levels of adhesion were inhibited significantly by transient expression of GnT-V, confirming the hypothesis that levels of GnT-V can regulate cadherin-associated homotypic cell-cell adhesion. Aberrant N-linked beta(1,6) branching that occurs during oncogenesis can, therefore, lessen cell-cell adhesion, contributing to increased cellular motility and invasiveness."xsd:string
http://purl.uniprot.org/citations/14561752http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m308837200"xsd:string
http://purl.uniprot.org/citations/14561752http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m308837200"xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/author"Lee I."xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/author"Lee I."xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/author"Pierce M."xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/author"Pierce M."xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/author"Guo H.B."xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/author"Guo H.B."xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/author"Kamar M."xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/author"Kamar M."xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/date"2003"xsd:gYear
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/date"2003"xsd:gYear
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/pages"52412-52424"xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/pages"52412-52424"xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/title"N-acetylglucosaminyltransferase V expression levels regulate cadherin-associated homotypic cell-cell adhesion and intracellular signaling pathways."xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/title"N-acetylglucosaminyltransferase V expression levels regulate cadherin-associated homotypic cell-cell adhesion and intracellular signaling pathways."xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/volume"278"xsd:string
http://purl.uniprot.org/citations/14561752http://purl.uniprot.org/core/volume"278"xsd:string
http://purl.uniprot.org/citations/14561752http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/14561752
http://purl.uniprot.org/citations/14561752http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/14561752
▶