| http://purl.uniprot.org/citations/14565952 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14565952 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14565952 | http://www.w3.org/2000/01/rdf-schema#comment | "Serum response factor (SRF) is required for the expression of a wide variety of muscle-specific genes that are expressed upon differentiation and is thus required for both striated and smooth muscle differentiation in addition to its role in regulating growth factor-inducible genes. A heart and smooth muscle-specific SRF co-activator, myocardin, has been shown to be required for cardiac development and smooth muscle differentiation. However, no such co-factors of SRF have been identified in the skeletal myogenic differentiation program. Myocardin and the related transcription factor megakaryoblastic leukemia-1 (MKL1/MAL/MRTF-A) can strongly potentiate the activity of SRF. Here we report the cloning of the third member of the myocardin/MKL family in humans, MKL2. MKL2 binds to and activates SRF similar to myocardin and MKL1. To determine the role of these factors in skeletal myogenic differentiation we used a dominant negative MKL2 to show that the MKL family of proteins is required for skeletal myogenic differentiation. Expression of the dominant negative protein in C2C12 skeletal myoblasts blocked the differentiation-induced expression of the SRF target genes skeletal alpha-actin and alpha-myosin heavy chain and blocked differentiation of the myoblasts to myotubes in vitro. C2C12 cells express both MKL1 and MKL2, but not myocardin, implicating MKL1 and/or MKL2 in the requirement for skeletal myogenic differentiation. MKL1 was predominantly cytoplasmic in C2C12 cells, with a small amount in the nucleus, however, no movement of MKL1 to the nucleus was observed upon differentiation."xsd:string |
| http://purl.uniprot.org/citations/14565952 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m305679200"xsd:string |
| http://purl.uniprot.org/citations/14565952 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m305679200"xsd:string |
| http://purl.uniprot.org/citations/14565952 | http://purl.uniprot.org/core/author | "Prywes R."xsd:string |
| http://purl.uniprot.org/citations/14565952 | http://purl.uniprot.org/core/author | "Prywes R."xsd:string |
| http://purl.uniprot.org/citations/14565952 | http://purl.uniprot.org/core/author | "Selvaraj A."xsd:string |
| http://purl.uniprot.org/citations/14565952 | http://purl.uniprot.org/core/author | "Selvaraj A."xsd:string |
| http://purl.uniprot.org/citations/14565952 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14565952 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14565952 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/14565952 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/14565952 | http://purl.uniprot.org/core/pages | "41977-41987"xsd:string |
| http://purl.uniprot.org/citations/14565952 | http://purl.uniprot.org/core/pages | "41977-41987"xsd:string |
| http://purl.uniprot.org/citations/14565952 | http://purl.uniprot.org/core/title | "Megakaryoblastic leukemia-1/2, a transcriptional co-activator of serum response factor, is required for skeletal myogenic differentiation."xsd:string |
| http://purl.uniprot.org/citations/14565952 | http://purl.uniprot.org/core/title | "Megakaryoblastic leukemia-1/2, a transcriptional co-activator of serum response factor, is required for skeletal myogenic differentiation."xsd:string |
| http://purl.uniprot.org/citations/14565952 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/14565952 | http://purl.uniprot.org/core/volume | "278"xsd:string |
| http://purl.uniprot.org/citations/14565952 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14565952 |
| http://purl.uniprot.org/citations/14565952 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14565952 |
| http://purl.uniprot.org/citations/14565952 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/14565952 |
| http://purl.uniprot.org/citations/14565952 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/14565952 |
| http://purl.uniprot.org/embl-cds/AAQ82435.1 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/14565952 |
| http://purl.uniprot.org/uniprot/Q969V6 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/14565952 |