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| Subject | Predicate | Object |
|---|---|---|
| http://purl.uniprot.org/citations/14573650 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14573650 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14573650 | http://www.w3.org/2000/01/rdf-schema#comment | "Enterohemorrhagic Escherichia coli (EHEC) is a prominent, food-borne cause of diarrhea, bloody diarrhea, and the hemolytic uremic syndrome in industrialized countries. Most strains of EHEC carry the locus for enterocyte effacement (LEE) pathogenicity island, but a proportion of isolates from patients with severe disease do not carry LEE and very little is known about virulence factors in these organisms. LEE-negative strains of EHEC typically express Shiga toxin 2 and carry a large plasmid that encodes the production of EHEC hemolysin. In this study, we determined the nucleotide sequence of the transfer region of pO113, the large hemolysin plasmid from LEE-negative EHEC O113:H21 (EH41). This 63.9-kb region showed a high degree of similarity with the transfer region of R64, and pO113 was capable of self-transmission at low frequencies. Unlike R64 and the related dot/icm system of Legionella pneumophila, however, pO113 was unable to mobilize RSF1010. In addition, the pO113 transfer region encoded a novel high-molecular-weight serine protease autotransporter of Enterobacteriaceae (SPATE) protein, termed EpeA. Like other SPATEs, EpeA exhibited protease activity and mucinase activity, but expression was not associated with a cytopathic effect on epithelial cells. Analysis of a second high-molecular-weight secreted protein revealed that pO113 also encodes EspP, a cytopathic SPATE identified previously in EHEC O157:H7. The nucleotide sequences encoding the predicted beta-domains of espP and epeA were identical and also shared significant homology with a third SPATE protein, EspI. Both espP and epeA were detected in several LEE-negative clinical isolates of EHEC and thus may contribute to the pathogenesis of this subset of EHEC."xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.org/dc/terms/identifier | "doi:10.1128/IAI.71.11.6307-6319.2003"xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.org/dc/terms/identifier | "doi:10.1128/iai.71.11.6307-6319.2003"xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/author | "Hill R.E."xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/author | "Hill R.E."xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/author | "Sloan J."xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/author | "Sloan J."xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/author | "Hartland E.L."xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/author | "Hartland E.L."xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/author | "Leyton D.L."xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/author | "Leyton D.L."xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/author | "Doughty S."xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/author | "Doughty S."xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/name | "Infect. Immun."xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/name | "Infect Immun"xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/pages | "6307-6319"xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/pages | "6307-6319"xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/title | "Transfer region of pO113 from enterohemorrhagic Escherichia coli: similarity with R64 and identification of a novel plasmid-encoded autotransporter, EpeA."xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/title | "Transfer region of pO113 from enterohemorrhagic Escherichia coli: similarity with R64 and identification of a novel plasmid-encoded autotransporter, EpeA."xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/volume | "71"xsd:string |
| http://purl.uniprot.org/citations/14573650 | http://purl.uniprot.org/core/volume | "71"xsd:string |