| http://purl.uniprot.org/citations/14585983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14585983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14585983 | http://www.w3.org/2000/01/rdf-schema#comment | "Substrates of the ubiquitin-dependent N-end rule pathway include proteins with destabilizing N-terminal residues. UBR1(-/-) mice, which lacked the pathway's ubiquitin ligase E3alpha, were viable and retained the N-end rule pathway. The present work describes the identification and analysis of mouse UBR2, a homolog of UBR1. We demonstrate that the substrate-binding properties of UBR2 are highly similar to those of UBR1, identifying UBR2 as the second E3 of the mammalian N-end rule pathway. UBR2(-/-) mouse strains were constructed, and their viability was found to be dependent on both gender and genetic background. In the strain 129 (inbred) background, the UBR2(-/-) genotype was lethal to most embryos of either gender. In the 129/B6 (mixed) background, most UBR2(-/-) females died as embryos, whereas UBR2(-/-) males were viable but infertile, owing to the postnatal degeneration of the testes. The gross architecture of UBR2(-/-) testes was normal and spermatogonia were intact as well, but UBR2(-/-) spermatocytes were arrested between leptotene/zygotene and pachytene and died through apoptosis. A conspicuous defect of UBR2(-/-) spermatocytes was the absence of intact synaptonemal complexes. We conclude that the UBR2 ubiquitin ligase and, hence, the N-end rule pathway are required for male meiosis and spermatogenesis and for an essential aspect of female embryonic development."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.23.22.8255-8271.2003"xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.23.22.8255-8271.2003"xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Xie Y."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Xie Y."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Sheng J."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Sheng J."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Xia Z."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Xia Z."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Varshavsky A."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Varshavsky A."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Kwon Y.T."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Kwon Y.T."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Seo J.W."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Seo J.W."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Davydov I.V."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Davydov I.V."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Tasaki T."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "Tasaki T."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "An J.Y."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/author | "An J.Y."xsd:string |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14585983 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |