| http://purl.uniprot.org/citations/14596619 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14596619 | http://www.w3.org/2000/01/rdf-schema#comment | "Polycystin-1, the protein product of the polycystic kidney disease-1 (PKD1) gene, was originally predicted to be an integral membrane glycoprotein with 11 transmembrane (TM) domains (TM 1-11). Subsequent comparative sequence analyses led to a revision of the original model, which retained the overall topology and 11 TM segments (TM I-XI) but dropped 3 of the original domains and introduced 3 new TM domains. The membrane-spanning potential and the orientation of each of the proposed TM domains following the extracellular REJ domain (TM I-XI and TM 11) have now been tested. Using a series of N-terminal polycystin TM-glycosylation reporter gene fusions expressed in vivo, we assayed N-linked glycosylation of the C-terminal glycosylation reporter as an indicator of TM domain presence and orientation. This approach has clearly demonstrated that 7 of the 12 TM domains tested function as membrane-spanning domains. In vitro analysis of the topogenic potential of the five remaining TM domains revealed that four of these also function as membrane-spanning domains, thus supporting an 11 TM structure for polycystin-1 comprised of TM domains I-XI. In addition, these studies suggest that the membrane insertion of TM domains I-IX occurs in a cotranslational and sequential manner, while multiple topogenic determinants appear to be required for the integration of the C-terminal-most TM segments of polycystin-1."xsd:string |
| http://purl.uniprot.org/citations/14596619 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi035074c"xsd:string |
| http://purl.uniprot.org/citations/14596619 | http://purl.uniprot.org/core/author | "Maser R.L."xsd:string |
| http://purl.uniprot.org/citations/14596619 | http://purl.uniprot.org/core/author | "Vassmer D."xsd:string |
| http://purl.uniprot.org/citations/14596619 | http://purl.uniprot.org/core/author | "Nims N."xsd:string |
| http://purl.uniprot.org/citations/14596619 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14596619 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/14596619 | http://purl.uniprot.org/core/pages | "13035-13048"xsd:string |
| http://purl.uniprot.org/citations/14596619 | http://purl.uniprot.org/core/title | "Transmembrane domain analysis of polycystin-1, the product of the polycystic kidney disease-1 (PKD1) gene: evidence for 11 membrane-spanning domains."xsd:string |
| http://purl.uniprot.org/citations/14596619 | http://purl.uniprot.org/core/volume | "42"xsd:string |
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