| http://purl.uniprot.org/citations/14630934 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14630934 | http://www.w3.org/2000/01/rdf-schema#comment | "In contrast to batch cultivation, chemostat cultivation allows the identification of carbon source responses without interference by carbon-catabolite repression, accumulation of toxic products, and differences in specific growth rate. This study focuses on the yeast Saccharomyces cerevisiae, grown in aerobic, carbon-limited chemostat cultures. Genome-wide transcript levels and in vivo fluxes were compared for growth on two sugars, glucose and maltose, and for two C2-compounds, ethanol and acetate. In contrast to previous reports on batch cultures, few genes (180 genes) responded to changes of the carbon source by a changed transcript level. Very few transcript levels were changed when glucose as the growth-limiting nutrient was compared with maltose (33 transcripts), or when acetate was compared with ethanol (16 transcripts). Although metabolic flux analysis using a stoichiometric model revealed major changes in the central carbon metabolism, only 117 genes exhibited a significantly different transcript level when sugars and C2-compounds were provided as the growth-limiting nutrient. Despite the extensive knowledge on carbon source regulation in yeast, many of the carbon source-responsive genes encoded proteins with unknown or incompletely characterized biological functions. In silico promoter analysis of carbon source-responsive genes confirmed the involvement of several known transcriptional regulators and suggested the involvement of additional regulators. Transcripts involved in the glyoxylate cycle and gluconeogenesis showed a good correlation with in vivo fluxes. This correlation was, however, not observed for other important pathways, including the pentose-phosphate pathway, tricarboxylic acid cycle, and, in particular, glycolysis. These results indicate that in vivo fluxes in the central carbon metabolism of S. cerevisiae grown in steadystate, carbon-limited chemostat cultures are controlled to a large extent via post-transcriptional mechanisms."xsd:string |
| http://purl.uniprot.org/citations/14630934 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m309578200"xsd:string |
| http://purl.uniprot.org/citations/14630934 | http://purl.uniprot.org/core/author | "Daran-Lapujade P."xsd:string |
| http://purl.uniprot.org/citations/14630934 | http://purl.uniprot.org/core/author | "Pronk J.T."xsd:string |
| http://purl.uniprot.org/citations/14630934 | http://purl.uniprot.org/core/author | "de Winde J.H."xsd:string |
| http://purl.uniprot.org/citations/14630934 | http://purl.uniprot.org/core/author | "Daran J.M."xsd:string |
| http://purl.uniprot.org/citations/14630934 | http://purl.uniprot.org/core/author | "Jansen M.L."xsd:string |
| http://purl.uniprot.org/citations/14630934 | http://purl.uniprot.org/core/author | "van Gulik W."xsd:string |
| http://purl.uniprot.org/citations/14630934 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14630934 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/14630934 | http://purl.uniprot.org/core/pages | "9125-9138"xsd:string |
| http://purl.uniprot.org/citations/14630934 | http://purl.uniprot.org/core/title | "Role of transcriptional regulation in controlling fluxes in central carbon metabolism of Saccharomyces cerevisiae. A chemostat culture study."xsd:string |
| http://purl.uniprot.org/citations/14630934 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/14630934 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14630934 |
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| http://purl.uniprot.org/uniprot/P15685 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/14630934 |
| http://purl.uniprot.org/uniprot/P07265 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/14630934 |