http://purl.uniprot.org/citations/14630966 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/14630966 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
http://purl.uniprot.org/citations/14630966 | http://www.w3.org/2000/01/rdf-schema#comment | "We report the identification and biotechnological utility of a plant gene encoding the tocopherol (vitamin E) biosynthetic enzyme 2-methyl-6-phytylbenzoquinol methyltransferase. This gene was identified by map-based cloning of the Arabidopsis mutation vitamin E pathway gene3-1 (vte3-1), which causes increased accumulation of delta-tocopherol and decreased gamma-tocopherol in the seed. Enzyme assays of recombinant protein supported the hypothesis that At-VTE3 encodes a 2-methyl-6-phytylbenzoquinol methyltransferase. Seed-specific expression of At-VTE3 in transgenic soybean reduced seed delta-tocopherol from 20 to 2%. These results confirm that At-VTE3 protein catalyzes the methylation of 2-methyl-6-phytylbenzoquinol in planta and show the utility of this gene in altering soybean tocopherol composition. When At-VTE3 was coexpressed with At-VTE4 (gamma-tocopherol methyltransferase) in soybean, the seed accumulated to >95% alpha-tocopherol, a dramatic change from the normal 10%, resulting in a greater than eightfold increase of alpha-tocopherol and an up to fivefold increase in seed vitamin E activity. These findings demonstrate the utility of a gene identified in Arabidopsis to alter the tocopherol composition of commercial seed oils, a result with both nutritional and food quality implications."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.org/dc/terms/identifier | "doi:10.1105/tpc.015875"xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/author | "Jiang J."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/author | "Last R.L."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/author | "Stein J.C."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/author | "Valentin H.E."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/author | "Durrett T.P."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/author | "Hao M."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/author | "Shewmaker C.K."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/author | "Baszis S.R."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/author | "Lincoln K."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/author | "Norris S.R."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/author | "Levering C.K."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/author | "Van Eenennaam A.L."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/author | "Aasen E.D."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/author | "Thorne G.M."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/name | "Plant Cell"xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/pages | "3007-3019"xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/title | "Engineering vitamin E content: from Arabidopsis mutant to soy oil."xsd:string |
http://purl.uniprot.org/citations/14630966 | http://purl.uniprot.org/core/volume | "15"xsd:string |
http://purl.uniprot.org/citations/14630966 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14630966 |
http://purl.uniprot.org/citations/14630966 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14630966 |