| http://purl.uniprot.org/citations/14633974 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14633974 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14633974 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/14633974 | http://www.w3.org/2000/01/rdf-schema#comment | "All tRNAHis molecules are unusual in having an extra 5' GMP residue (G(-1)) that, in eukaryotes, is added after transcription and RNase P cleavage. Incorporation of this G(-1) residue is a rare example of nucleotide addition occurring at an RNA 5' end in a normal phosphodiester linkage. We show here that the essential Saccharomyces cerevisiae ORF YGR024c (THG1) is responsible for this guanylyltransferase reaction. Thg1p was identified by survey of a genomic collection of yeast GST-ORF fusion proteins for addition of [alpha-32P]GTP to tRNAHis. End analysis confirms the presence of G(-1). Thg1p is required for tRNAHis guanylylation in vivo, because cells depleted of Thg1p lack G(-1) in their tRNAHis. His6-Thg1p purified from Escherichia coli catalyzes the guanylyltransferase step of G(-1) addition using a ppp-tRNAHis substrate, and appears to catalyze the activation step using p-tRNAHis and ATP. Thg1p is highlye conserved in eukaryotes, where G(-1) addition is necessary, and is not found in eubacteria, where G(-1) is genome-encoded. Thus, Thg1p is the first member of a new family of enzymes that can catalyze phosphodiester bond formation at the 5' end of RNAs, formally in a 3'-5' direction. Surprisingly, despite its varied activities, Thg1p contains no recognizable catalytic or functional domains."xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.1148603"xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.org/dc/terms/identifier | "doi:10.1101/gad.1148603"xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/author | "Gray M.W."xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/author | "Gray M.W."xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/author | "Gu W."xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/author | "Gu W."xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/author | "Phizicky E.M."xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/author | "Phizicky E.M."xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/author | "Jackman J.E."xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/author | "Jackman J.E."xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/author | "Lohan A.J."xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/author | "Lohan A.J."xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/name | "Genes Dev."xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/pages | "2889-2901"xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/pages | "2889-2901"xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/title | "tRNAHis maturation: an essential yeast protein catalyzes addition of a guanine nucleotide to the 5' end of tRNAHis."xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/title | "tRNAHis maturation: an essential yeast protein catalyzes addition of a guanine nucleotide to the 5' end of tRNAHis."xsd:string |
| http://purl.uniprot.org/citations/14633974 | http://purl.uniprot.org/core/volume | "17"xsd:string |