| http://purl.uniprot.org/citations/14662325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14662325 | http://www.w3.org/2000/01/rdf-schema#comment | "ObjectiveTo determine the role of the c-terminal half of c-Mpl in Mpl-L-induced myeloprotection and the importance of Stat5 isoforms in the survival signaling pathways induced by Mpl ligand.Materials and methodsDelta60-Mpl knockin mice, Stat5a(-/-)/b(-/-), Stat5a(-/-), and Stat5b(-/-) mice and wild-type (WT) controls were given a lethal myelosuppressive regimen: 80 mg/kg carboplatin intravenously followed by 7.5 or 6.5 Gy 137Cs total-body irradiation. A single dose of PEG-rmMGDF (65 microg/kg) was intravenously injected immediately after myelosuppression. Mice survival and blood counts were monitored for 22 days posttreatment.ResultsKnockin Delta60-Mpl mice lacking the c-terminal half of the intracellular domain of c-Mpl show reduced ability of Mpl-L to prevent lethal myelosuppression and an impaired thrombopoietic response to exogenous c-Mpl ligand. The survival of Mpl-L-treated Stat5a(-/-)/b(-/-) mice exposed to the lethal myelosuppressive regimen was substantially compromised compared to that of WT mice. Reduced survival of Stat5a(-/-)/b(-/-) mice was due to more severe hematopoietic suppression. Deletion of Stat5a did not result in a defect in hematopoietic recovery. In contrast, Mpl-L-treated Stat5b-deficient mice demonstrated significantly delayed hematopoietic recovery compared to WT controls.ConclusionsMyeloprotective signaling transduced by the terminal 60 amino acids of the intracellular domain of c-Mpl is essential for complete protection from lethal myelosuppression provided by Mpl-L. Our studies differentiate the functions of Stat5 isoforms in hematopoietic stress and reveal a pivotal role of Stat5b in Mpl-L-induced hematopoietic recovery in this lethal myelosuppression model."xsd:string |
| http://purl.uniprot.org/citations/14662325 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.exphem.2003.09.002"xsd:string |
| http://purl.uniprot.org/citations/14662325 | http://purl.uniprot.org/core/author | "Jackson C.W."xsd:string |
| http://purl.uniprot.org/citations/14662325 | http://purl.uniprot.org/core/author | "Pestina T.I."xsd:string |
| http://purl.uniprot.org/citations/14662325 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14662325 | http://purl.uniprot.org/core/name | "Exp Hematol"xsd:string |
| http://purl.uniprot.org/citations/14662325 | http://purl.uniprot.org/core/pages | "1198-1205"xsd:string |
| http://purl.uniprot.org/citations/14662325 | http://purl.uniprot.org/core/title | "Differential role of Stat5 isoforms in effecting hematopoietic recovery induced by Mpl-ligand in lethally myelosuppressed mice."xsd:string |
| http://purl.uniprot.org/citations/14662325 | http://purl.uniprot.org/core/volume | "31"xsd:string |
| http://purl.uniprot.org/citations/14662325 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14662325 |
| http://purl.uniprot.org/citations/14662325 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/14662325 |
| http://purl.uniprot.org/uniprot/#_E9Q7U5-mappedCitation-14662325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14662325 |
| http://purl.uniprot.org/uniprot/#_A2A5D3-mappedCitation-14662325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14662325 |
| http://purl.uniprot.org/uniprot/#_A2A5D4-mappedCitation-14662325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14662325 |
| http://purl.uniprot.org/uniprot/#_A2A9D4-mappedCitation-14662325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14662325 |
| http://purl.uniprot.org/uniprot/#_A6QL55-mappedCitation-14662325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14662325 |
| http://purl.uniprot.org/uniprot/#_B2C3G8-mappedCitation-14662325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14662325 |
| http://purl.uniprot.org/uniprot/#_Q71UV3-mappedCitation-14662325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14662325 |
| http://purl.uniprot.org/uniprot/#_P51692-mappedCitation-14662325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14662325 |
| http://purl.uniprot.org/uniprot/#_Q08351-mappedCitation-14662325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14662325 |
| http://purl.uniprot.org/uniprot/#_Q3ZB68-mappedCitation-14662325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14662325 |
| http://purl.uniprot.org/uniprot/#_Q3ZB69-mappedCitation-14662325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14662325 |
| http://purl.uniprot.org/uniprot/#_Q3UZ79-mappedCitation-14662325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14662325 |
| http://purl.uniprot.org/uniprot/#_Q925F5-mappedCitation-14662325 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14662325 |