| http://purl.uniprot.org/citations/14667819 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14667819 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14667819 | http://www.w3.org/2000/01/rdf-schema#comment | "High-throughput (HTP) protein-interaction assays, such as the yeast two-hybrid (Y2H) system, are enormously useful in predicting the functions of novel gene-products. HTP-Y2H screens typically do not include all of the reconfirmation and specificity tests used in small-scale studies, but the effects of omitting these steps have not been assessed. We performed HTP-Y2H screens that included all standard controls, using the predicted intracellular proteins expressed from the human MHC class III region, a region of the genome associated with many autoimmune diseases. The 91 novel interactions identified provide insight into the potential functions of many MHC genes, including C6orf47, LSM2, NELF-E (RDBP), DOM3Z, STK19, PBX2, RNF5, UAP56 (BAT1), ATP6G2, LST1/f, BAT2, Scythe (BAT3), CSNK2B, BAT5, and CLIC1. Surprisingly, our results predict that 1/3 of the proteins may have a role in mRNA processing, which suggests clustering of functionally related genes within the human genome. Most importantly, our analysis shows that omitting standard controls in HTP-Y2H screens could significantly compromise data quality."xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0888-7543(03)00235-0"xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0888-7543(03)00235-0"xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/author | "Brown S.E."xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/author | "Brown S.E."xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/author | "Campbell R.D."xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/author | "Campbell R.D."xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/author | "Sanderson C.M."xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/author | "Sanderson C.M."xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/author | "Counsell D."xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/author | "Counsell D."xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/author | "Lehner B."xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/author | "Lehner B."xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/author | "Semple J.I."xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/author | "Semple J.I."xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/name | "Genomics"xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/name | "Genomics"xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/pages | "153-167"xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/pages | "153-167"xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/title | "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region."xsd:string |
| http://purl.uniprot.org/citations/14667819 | http://purl.uniprot.org/core/title | "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region."xsd:string |