| http://purl.uniprot.org/citations/14668479 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14668479 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14668479 | http://www.w3.org/2000/01/rdf-schema#comment | "Our previous work found the two yeast plasma membrane-localized casein kinases Yck1p and Yck2p to be palmitoylated on C-terminal Cys-Cys sequences by the palmitoyl transferase Akr1p. The present work examines a third casein kinase, Yck3p, which ends with the C-terminal sequence Cys-Cys-Cys-Cys-Phe-Cys-Cys-Cys. Yck3p is palmitoylated and localized to the vacuolar membrane. While the C-terminal cysteines are required for this palmitoylation, Akr1p is not. Palmitoylation requires the C-terminal Yck3p residues 463-524, whereas information for vacuolar sorting maps to the 409-462 interval. Vacuolar sorting is disrupted in cis through deletion of the 409-462 sequences and in trans through mutation of the AP-3 adaptin complex; both cis- and trans-mutations result in Yck3p missorting to the plasma membrane. This missorted Yck3p restores 37 degrees C viability to yck1Delta yck2-ts cells. yck1Delta yck2-ts suppressor mutations isolated within the YCK3 gene identify the Yck3p vacuolar sorting signal-the tetrapeptide YDSI, a perfect fit to the YXXPhi adaptin-binding consensus. Although YXXPhi signals have a well-appreciated role in the adaptin-mediated sorting of mammalian cells, this is the first signal of this class to be identified in yeast."xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e03-09-0682"xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e03-09-0682"xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/author | "Chen L."xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/author | "Chen L."xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/author | "Sun B."xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/author | "Sun B."xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/author | "Cao W."xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/author | "Cao W."xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/author | "Davis N.G."xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/author | "Davis N.G."xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/author | "Roth A.F."xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/author | "Roth A.F."xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/name | "Mol. Biol. Cell"xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/name | "Mol. Biol. Cell"xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/pages | "1397-1406"xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/pages | "1397-1406"xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/title | "The yeast casein kinase Yck3p is palmitoylated, then sorted to the vacuolar membrane with AP-3-dependent recognition of a YXXPhi adaptin sorting signal."xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/title | "The yeast casein kinase Yck3p is palmitoylated, then sorted to the vacuolar membrane with AP-3-dependent recognition of a YXXPhi adaptin sorting signal."xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/volume | "15"xsd:string |
| http://purl.uniprot.org/citations/14668479 | http://purl.uniprot.org/core/volume | "15"xsd:string |