| http://purl.uniprot.org/citations/14673090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14673090 | http://www.w3.org/2000/01/rdf-schema#comment | "Mitochondrial cytochrome c oxidase plays an essential role in aerobic cellular respiration, reducing dioxygen to water in a process coupled with the pumping of protons across the mitochondrial inner membrane. An aspartate residue, Asp-51, located near the enzyme surface, undergoes a redox-coupled x-ray structural change, which is suggestive of a role for this residue in redox-driven proton pumping. However, functional or mechanistic evidence for the involvement of this residue in proton pumping has not yet been obtained. We report that the Asp-51 --> Asn mutation of the bovine enzyme abolishes its proton-pumping function without impairment of the dioxygen reduction activity. Improved x-ray structures (at 1.8/1.9-A resolution in the fully oxidized/reduced states) show that the net positive charge created upon oxidation of the low-spin heme of the enzyme drives the active proton transport from the interior of the mitochondria to Asp-51 across the enzyme via a water channel and a hydrogen-bond network, located in tandem, and that the enzyme reduction induces proton ejection from the aspartate to the mitochondrial exterior. A peptide bond in the hydrogen-bond network critically inhibits reverse proton transfer through the network. A redox-coupled change in the capacity of the water channel, induced by the hydroxyfarnesylethyl group of the low-spin heme, suggests that the channel functions as an effective proton-collecting region. Infrared results indicate that the conformation of Asp-51 is controlled only by the oxidation state of the low-spin heme. These results indicate that the low-spin heme drives the proton-pumping process."xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.2635097100"xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/author | "Katayama Y."xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/author | "Mochizuki M."xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/author | "Tsukihara T."xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/author | "Shimada H."xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/author | "Yao M."xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/author | "Yamashita E."xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/author | "Muramoto K."xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/author | "Yoshikawa S."xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/author | "Ishimura Y."xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/author | "Shimokata K."xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/author | "Aoyama H."xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/author | "Shinzawa-Itoh K."xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/date | "2003"xsd:gYear |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/pages | "15304-15309"xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/title | "The low-spin heme of cytochrome c oxidase as the driving element of the proton-pumping process."xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://purl.uniprot.org/core/volume | "100"xsd:string |
| http://purl.uniprot.org/citations/14673090 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14673090 |
| http://purl.uniprot.org/citations/14673090 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/14673090 |
| http://purl.uniprot.org/uniprot/#_P13183-mappedCitation-14673090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14673090 |
| http://purl.uniprot.org/uniprot/#_P10175-mappedCitation-14673090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14673090 |
| http://purl.uniprot.org/uniprot/#_P00396-mappedCitation-14673090 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14673090 |