| http://purl.uniprot.org/citations/14676198 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14676198 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14676198 | http://www.w3.org/2000/01/rdf-schema#comment | "N-WASP is a member of the WASP family of proteins that regulate actin cytoskeleton remodeling. FAK is a cytoplasmic tyrosine kinase implicated in integrin signaling during cell migration. Here we identify a direct interaction between N-WASP and FAK and show that N-WASP is phosphorylated by FAK at a conserved tyrosine residue, Tyr(256). We found that phosphorylation of Tyr(256) affected N-WASP nuclear localization, suggesting that phosphorylation of N-WASP by FAK may regulate its activity in vivo by altering its subcellular localization. We also showed that the nuclear localization of N-WASP is dependent on its being in the open conformation either after its activation by Cdc42 or the truncation of the C-terminal VCA domain. Phosphorylation of Tyr(256) of N-WASP could reduce its interaction with nuclear importin NPI-1, which might be responsible for its decreased nuclear localization. Lastly, we show that phosphorylation of Tyr(256) plays an important role in promoting cell migration. Together, these results suggest a novel regulatory mechanism of N-WASP by tyrosine phosphorylation and subcellular localization and its potential role in the regulation of cell migration."xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m310739200"xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m310739200"xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/author | "Wu X."xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/author | "Wu X."xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/author | "Suetsugu S."xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/author | "Suetsugu S."xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/author | "Takenawa T."xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/author | "Takenawa T."xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/author | "Cooper L.A."xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/author | "Cooper L.A."xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/author | "Guan J.L."xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/author | "Guan J.L."xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/pages | "9565-9576"xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/pages | "9565-9576"xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/title | "Focal adhesion kinase regulation of N-WASP subcellular localization and function."xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/title | "Focal adhesion kinase regulation of N-WASP subcellular localization and function."xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/14676198 | http://purl.uniprot.org/core/volume | "279"xsd:string |