| http://purl.uniprot.org/citations/14676825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14676825 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14676825 | http://www.w3.org/2000/01/rdf-schema#comment | "Processing of the NF-kappa B2 precursor p100 to the mature p52 subunit is regulated via a unique pathway. NF-kappa B-inducing kinase (NIK) induces I kappa B kinase alpha (IKK alpha)-mediated phosphorylation of specific serine residues in the C-terminal domain of p100, leading to recruitment of the SCF(beta-TrCP) ubiquitin ligase. We identified a single lysine residue, K855, that serves as the ubiquitin-anchoring residue required for signal-induced processing of p100. In a reconstituted system containing purified components, p100-K855R could not be ubiquitinated. In a crude extract and cells, only residual, signal-independent ubiquitination and processing were retained. Importantly, K855 is located in a site homologous to K22 that serves as an ubiquitination site in I kappa B alpha. This suggests a common recognition mechanism for the two molecules. In contrast, p105, the p100 homologue, lacks a similar Lys residue. We also demonstrate that the NEDD8 pathway is essential for the SCF(beta-TrCP) activity. In a reconstituted system, efficient ubiquitination of p100 required all three components of the pathway - E1, the UBC12 E2 and NEDD8. Experiments in reconstituted systems and in cells demonstrated that SCF(beta-TrCP), which contains a mutant Cul-1 that cannot be NEDDylated, cannot stimulate ubiquitination and processing. Similarly, dominant negative UBC12 inhibits, in a reversible manner, both ubiquitination and processing of p100."xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.onc.1207366"xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.org/dc/terms/identifier | "doi:10.1038/sj.onc.1207366"xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/author | "Ciechanover A."xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/author | "Ciechanover A."xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/author | "Karin M."xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/author | "Karin M."xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/author | "Amir R.E."xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/author | "Amir R.E."xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/author | "Haecker H."xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/author | "Haecker H."xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/name | "Oncogene"xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/name | "Oncogene"xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/pages | "2540-2547"xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/pages | "2540-2547"xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/title | "Mechanism of processing of the NF-kappa B2 p100 precursor: identification of the specific polyubiquitin chain-anchoring lysine residue and analysis of the role of NEDD8-modification on the SCF(beta-TrCP) ubiquitin ligase."xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/title | "Mechanism of processing of the NF-kappa B2 p100 precursor: identification of the specific polyubiquitin chain-anchoring lysine residue and analysis of the role of NEDD8-modification on the SCF(beta-TrCP) ubiquitin ligase."xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/14676825 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14676825 |
| http://purl.uniprot.org/citations/14676825 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14676825 |