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| http://purl.uniprot.org/citations/14684748 | http://www.w3.org/2000/01/rdf-schema#comment | "Heat shock cognate protein 70 (Hsc70) serves nuclear transport of several proteins as a molecular chaperone. We have recently identified a novel variant of human Hsc70, heat shock cognate protein 54 (Hsc54), that lacks amino acid residues 464-616 in the protein binding and variable domains of Hsc70. In the present study, we examined nucleocytoplasmic localization of Hsc70 and Hsc54 by using green fluorescent protein (GFP) fusions. GFP-Hsc70 is localized in both the cytoplasm and the nucleus at 37 degrees C and accumulated into the nucleolus/nucleus after heat shock, whereas GFP-Hsc54 always remained exclusively in the cytoplasm under these conditions. Mutation studies indicated that 20 amino acid residues of nuclear localization-related signals, which are missing in Hsc54 but are retained in Hsc70, are required for proper nuclear localization of Hsc70. We further found that Hsc54 contains a functional leucine-rich nuclear export signal (NES, (394)LDVTPLSL(401)) which is differently situated from the previously proposed NES in Saccharomyces cerevisiae Ssb1p. The cytoplasmic localization of Hsc54 was impaired by a mutation in NES as well as by a nuclear export inhibitor, leptomycin B, suggesting that Hsc54 is actively exported from the nucleus to the cytoplasm through a CRM1-dependent mechanism. In contrast, the nucleocytoplasmic localization of Hsc70 was not affected by the same mutation of NES or leptomycin B. These results suggest that the nuclear localization-related signal could functionally mask NES leading to prolonged retention of Hsc70 in the nucleus. An additional mechanism for unmasking the NES may regulate nucleocytoplasmic trafficking of Hsc70."xsd:string |
| http://purl.uniprot.org/citations/14684748 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m308848200"xsd:string |
| http://purl.uniprot.org/citations/14684748 | http://purl.uniprot.org/core/author | "Tsukahara F."xsd:string |
| http://purl.uniprot.org/citations/14684748 | http://purl.uniprot.org/core/author | "Maru Y."xsd:string |
| http://purl.uniprot.org/citations/14684748 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14684748 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/14684748 | http://purl.uniprot.org/core/pages | "8867-8872"xsd:string |
| http://purl.uniprot.org/citations/14684748 | http://purl.uniprot.org/core/title | "Identification of novel nuclear export and nuclear localization-related signals in human heat shock cognate protein 70."xsd:string |
| http://purl.uniprot.org/citations/14684748 | http://purl.uniprot.org/core/volume | "279"xsd:string |
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