| http://purl.uniprot.org/citations/14688353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14688353 | http://www.w3.org/2000/01/rdf-schema#comment | "Thioredoxin (TRX) is released from various types of mammalian cells despite no typical secretory signal sequence. We show here that a redox-active site in TRX is essential for its release from T lymphocytes in response to H2O2 and extracellular TRX regulates its own H2O2-induced release. Human T cell leukemia virus type I-transformed T lymphocytes constitutively release a large amount of TRX. The level of TRX release is augmented upon the addition of H2O2, but suppressed upon the addition of N-acetylcysteine. In the culture supernatant of a Jurkat transfectant expressing the tagged TRX-wild type (WT), the tagged TRX protein is rapidly released at 1 h and kept at a constant level until 6 h after the addition of H2O2. In contrast, another type of transfectant expressing the tagged TRX mutant (C32S/C35S; CS) fails to release the protein. H2O2-induced release of TRX from the transfectant is inhibited by the presence of rTRX-WT in a dose-dependent manner. Preincubation of the transfectant with rTRX-WT for 1 h at 37 degrees C, but not 0 degrees C, results in a significant suppression of the TRX release, reactive oxygen species, and caspase-3 activity induced by H2O2, respectively. Confocal microscopy and Western blot analysis show that extracellular rTRX-WT added to the culture does not obviously enter T lymphocytes until 24 h. These results collectively suggest that the oxidative stress-induced TRX release from T lymphocytes depends on a redox-sensitive event and may be regulated by negative feedback loops using reactive oxygen species-mediated signal transductions."xsd:string |
| http://purl.uniprot.org/citations/14688353 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.172.1.442"xsd:string |
| http://purl.uniprot.org/citations/14688353 | http://purl.uniprot.org/core/author | "Ishii Y."xsd:string |
| http://purl.uniprot.org/citations/14688353 | http://purl.uniprot.org/core/author | "Nakamura H."xsd:string |
| http://purl.uniprot.org/citations/14688353 | http://purl.uniprot.org/core/author | "Kondo N."xsd:string |
| http://purl.uniprot.org/citations/14688353 | http://purl.uniprot.org/core/author | "Horita H."xsd:string |
| http://purl.uniprot.org/citations/14688353 | http://purl.uniprot.org/core/author | "Yodoi J."xsd:string |
| http://purl.uniprot.org/citations/14688353 | http://purl.uniprot.org/core/author | "Nishinaka Y."xsd:string |
| http://purl.uniprot.org/citations/14688353 | http://purl.uniprot.org/core/author | "Kwon Y.W."xsd:string |
| http://purl.uniprot.org/citations/14688353 | http://purl.uniprot.org/core/author | "Tanito M."xsd:string |
| http://purl.uniprot.org/citations/14688353 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14688353 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/14688353 | http://purl.uniprot.org/core/pages | "442-448"xsd:string |
| http://purl.uniprot.org/citations/14688353 | http://purl.uniprot.org/core/title | "Redox-sensing release of human thioredoxin from T lymphocytes with negative feedback loops."xsd:string |
| http://purl.uniprot.org/citations/14688353 | http://purl.uniprot.org/core/volume | "172"xsd:string |
| http://purl.uniprot.org/citations/14688353 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14688353 |
| http://purl.uniprot.org/citations/14688353 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/14688353 |
| http://purl.uniprot.org/uniprot/#_H9ZYJ2-mappedCitation-14688353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14688353 |
| http://purl.uniprot.org/uniprot/#_P10599-mappedCitation-14688353 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14688353 |
| http://purl.uniprot.org/uniprot/H9ZYJ2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/14688353 |
| http://purl.uniprot.org/uniprot/P10599 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/14688353 |