| http://purl.uniprot.org/citations/14693708 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/14693708 | http://www.w3.org/2000/01/rdf-schema#comment | "Islet amyloid polypeptide (IAPP) (amylin), the major component of islet amyloid, is produced by cleavage at the COOH- and NH(2)-termini of its precursor, proIAPP, likely by the beta-cell prohormone convertases (PC) 1/3 and PC2. Mice lacking PC2 can process proIAPP at its COOH-but not its NH(2)-terminal cleavage site, suggesting that PC1/3 is capable of initiating proIAPP cleavage at its COOH-terminus. To determine the precise role of PC1/3 in proIAPP processing, Western blot analysis was performed on islets isolated from mice lacking PC1/3 (PC1/3(-/-)). These islets contained not only fully processed IAPP as in PC1/3(+/+) islets, but also elevated levels of a COOH-terminally unprocessed intermediate form, suggesting impaired processing at the COOH-terminus. Next, GH3 cells that do not normally express proIAPP or detectable levels of PC1/3 or PC2 were cotransduced with adenoviruses expressing rat proIAPP and either PC2 or PC1/3. As expected, in GH3 cells transduced to express only proIAPP, no processing was observed. Coexpression of proIAPP and PC2 resulted in production of mature IAPP, whereas in cells that coexpressed proIAPP and PC1/3 only a 6-kDa intermediate was produced. We conclude that PC1/3 is important for processing of proIAPP at the COOH-terminus, but in its absence, PC2 can initiate complete processing of proIAPP to IAPP by cleaving the precursor at either its NH(2)- or COOH-terminal cleavage sites."xsd:string |
| http://purl.uniprot.org/citations/14693708 | http://purl.org/dc/terms/identifier | "doi:10.2337/diabetes.53.1.141"xsd:string |
| http://purl.uniprot.org/citations/14693708 | http://purl.uniprot.org/core/author | "Zhu X."xsd:string |
| http://purl.uniprot.org/citations/14693708 | http://purl.uniprot.org/core/author | "Steiner D.F."xsd:string |
| http://purl.uniprot.org/citations/14693708 | http://purl.uniprot.org/core/author | "Trigo-Gonzalez G."xsd:string |
| http://purl.uniprot.org/citations/14693708 | http://purl.uniprot.org/core/author | "Verchere C.B."xsd:string |
| http://purl.uniprot.org/citations/14693708 | http://purl.uniprot.org/core/author | "Halban P.A."xsd:string |
| http://purl.uniprot.org/citations/14693708 | http://purl.uniprot.org/core/author | "Rhodes C.J."xsd:string |
| http://purl.uniprot.org/citations/14693708 | http://purl.uniprot.org/core/author | "Marzban L."xsd:string |
| http://purl.uniprot.org/citations/14693708 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/14693708 | http://purl.uniprot.org/core/name | "Diabetes"xsd:string |
| http://purl.uniprot.org/citations/14693708 | http://purl.uniprot.org/core/pages | "141-148"xsd:string |
| http://purl.uniprot.org/citations/14693708 | http://purl.uniprot.org/core/title | "Role of beta-cell prohormone convertase (PC)1/3 in processing of pro-islet amyloid polypeptide."xsd:string |
| http://purl.uniprot.org/citations/14693708 | http://purl.uniprot.org/core/volume | "53"xsd:string |
| http://purl.uniprot.org/citations/14693708 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/14693708 |
| http://purl.uniprot.org/citations/14693708 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/14693708 |
| http://purl.uniprot.org/uniprot/#_A0A1Y7VJY7-mappedCitation-14693708 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14693708 |
| http://purl.uniprot.org/uniprot/#_P21661-mappedCitation-14693708 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14693708 |
| http://purl.uniprot.org/uniprot/#_Q32MU0-mappedCitation-14693708 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14693708 |
| http://purl.uniprot.org/uniprot/#_P12968-mappedCitation-14693708 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14693708 |
| http://purl.uniprot.org/uniprot/#_Q3UZV3-mappedCitation-14693708 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14693708 |
| http://purl.uniprot.org/uniprot/#_P63239-mappedCitation-14693708 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14693708 |
| http://purl.uniprot.org/uniprot/#_Q3TYM6-mappedCitation-14693708 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14693708 |
| http://purl.uniprot.org/uniprot/#_Q3TT48-mappedCitation-14693708 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/14693708 |